烟草科技2025，Vol.58Issue(7)：35-43,9.DOI:10.16135/j.issn1002-0861.2024.0745
烟草生长素受体基因家族鉴定及表达分析
Identification and expression analysis of auxin receptor gene family in Nicotiana tabacum
摘要
Abstract
To elucidate the resistance of NtTIR1/AFBs family genes to auxin herbicides and the mechanism of quinclorac phytotoxicity in tobacco,the tobacco auxin receptor gene family was identified and their gene expression patterns were analyzed.The results showed that there were ten NtTIR1/AFBs genes in Nicotiana tabacum.Subcellular localization analysis showed that five NtTIR1/AFBs localize in the nucleus,three in the cytoplasm,and two in the chloroplasts.Phylogenetic analysis revealed that NtTIR1/AFBs were evolved into four classes,while AtTIR1/AFBs in Arabidopsis thaliana into three classes.Protein domain analysis revealed the presence of conserved F-box and LRR domains predominantly in NtTIR1/AFBs.Furthermore,there were differences in the number of domains between NtTIR1/AFBs and AtTIR1/AFBs.MicroRNA target analysis identified putative miRNA binding sites within four NtTIR1/AFBs genes,with 20083 and 20090 harboring eight miRNA target sites each.Tissue expression analysis revealed that the expressions of 20081,20082,20088 and 20091 were relatively high in all tissues.Notably,gene expression analysis after quinclorac treatment showed that the expressions of 20085 and 20086 were highly up-regulated in tobacco roots.20082 had the highest up-regulated expression fold in tobacco leaves,while 20081 and 20090 had the highest down-regulated expression fold in tobacco leaves.关键词
烟草/生长素受体/二氯喹啉酸药害/基因家族Key words
Nicotiana tabacum/Auxin receptor/Quinclorac phytotoxicity/Gene family分类
轻工纺织引用本文复制引用
高军平,王帅斌,陈凯,罗朝鹏,吕婧,孙玉合,杨军,杨小年,谢小东,朱丽颖,李冰洁,李锋,黄平俊,何鑫玺,李泽锋..烟草生长素受体基因家族鉴定及表达分析[J].烟草科技,2025,58(7):35-43,9.基金项目
湖南中烟工业有限责任公司科技项目"烟草二氯喹啉酸类除草剂抗性基因筛选与利用"(KY2020YC0003)、"基于重离子辐射的K326突变体库创制与种质筛选"(KY2024YC0019) （KY2020YC0003）
中国烟草总公司烟草基因组计划与生物育种重大科技项目"烟草抗二氯喹啉酸稻田除草剂药害的突变基因克隆与功能研究"[110202101036(JY-13)] （JY-13）
湖南中烟-郑州烟草研究院烟草基因功能研究与利用联合实验室项目. （）
