眼科新进展2025，Vol.45Issue(8)：597-602,6.DOI:10.13389/j.cnki.rao.2025.0103
毛兰素通过HIPPO/YAP信号通路抑制高糖诱导的ARPE-19细胞上皮-间充质转化的作用机制
Erianin inhibits the high glucose-induced epithelial-mesenchymal transition of ARPE-19 cells through the HIPPO/YeS-associated protein signaling path-way
摘要
Abstract
Objective To explore the mechanism by which Erianin(ER)inhibits the high glucose(HG)-induced ep-ithelial-mesenchymal transition of ARPE-19 cells through the HIPPO/YeS-associated protein(YAP)signaling pathway.Methods The CCK-8 assay was used to determine the optimal glucose concentration(which was 50 mmol·L-1)for in-ducing the proliferation of ARPE-19 cells under HG conditions.The cytotoxic effects of different concentrations of ER(in-cluding 10,20,and 40 nmol·L-1)on ARPE-19 cells were evaluated.ARPE-19 cells were divided into the control group(cultured in a normal medium for 48 h),the HG group(cultured in a medium supplemented with 50 mumol·L-1 glucose for 48 h),and the HG `+low/medium/high ER groups(cultured in a medium with 50 mmol·L-1 glucose for 48 h after pre-treatment with 10,20,and 40 nmol·L-1 ER for 1 h,respectively).The CCK-8 assay was performed to assess the prolifera-tion rate of ARPE-19 cells.The scratch assay was used to evaluate cell migration activity and calculate the wound healing percentage.Immunofluorescence was employed to detect the intracellular expression of Vimentin in ARPE-19 cells.West-ern blot analysis was used to determine the intracellular expression levels of α-smooth muscle actin(α-SMA),neural cad-herin(N-Cadherin),epithelial cadherin(E-Cadherin),mammalian sterile 20-like kinase 1(MST1),large tumor suppressor kinase 1(LATS1),YAP,and phosphorylated YAP(P-YAP)in ARPE-19 cells.Results Compared with those in the con-trol group,the proliferation rate,the wound healing percentage,and the immunofluorescence intensity of Vimentin proteins in ARPE-19 cells in the HG group were significantly increased(all P＜0.01).The relative expression levels of α-SMA,N-Cadherin,and YAP proteins were significantly elevated while the relative expression levels of E-cadherin,P-YAP,MST1,and LATS1 proteins were significantly decreased in the HG group than those in the control group(all P＜0.01).ARPE-19 cells in the HG+low/medium/high ER groups exhibited a lower cell proliferation rate,a lower wound healing percentage,and weakened Vimentin fluorescence intensity than those in the HG group(all P＜0.05).The relative expression levels of YAP,α-SMA,and N-cadherin proteins were significantly reduced while the relative expression levels of E-cadherin and P-YAP were significantly increased in the HG+low/medium/high ER groups,compared with those in the HG group(all P＜0.05).The relative expression levels of MST1 and LATS1 proteins in the HG+medium/high ER groups were significantly higher than those in the HG group(both P＜0.05).Conclusion ER can inhibit the HG-induced epithelial-mesenchymal transition of ARPE-19 cells,which may be related to the regulation of the Hippo/YAP signaling pathway.关键词
毛兰素/高糖/人视网膜色素上皮细胞/上皮-间充质转化Key words
Erianin/high glucose/human retinal pigment epithelial cell/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
杨舵,庞东渤..毛兰素通过HIPPO/YAP信号通路抑制高糖诱导的ARPE-19细胞上皮-间充质转化的作用机制[J].眼科新进展,2025,45(8):597-602,6.基金项目
辽宁省自然科学基金项目(编号:2019-ZD-0601) （编号:2019-ZD-0601）
