疑难病杂志2025，Vol.24Issue(7)：820-825,837,7.DOI:10.3969/j.issn.1671-6450.2025.07.010
结直肠癌组织中CAPRIN1、PDPN表达与侵袭转移基因的相关性及预后意义
The correlation between CAPRIN1,PDPN expression and invasion and metastasis genes in colorectal cancer tissues and prognostic significance
摘要
Abstract
Objective To investigate the correlation and prognostic significance of cell cycle associated protein 1(CAPRIN1)and podoplanin(PDPN)expression with invasion and metastasis genes in colorectal cancer(CRC)tissues.Methods The cancer tissues and adjacent tissues of 132 patients with CRC admitted to the Department of Gastrointestinal Surgery,the Tenth Affiliated Hospital of Southern Medical University/Dongguan People's Hospital from January 2020 to September 2021 were selected.Real-time fluorescence quantitative PCR(qPCR)was used to detect the mRNA expression of CAPRIN1,PDPN,invasion and metastasis genes[N-cadherin(N-cad),E-cadherin(E-cad),vimentin(Vim)]in adjacent tissues and cancer tissues,and the protein expression of CAPRIN1 and PDPN was detected by immunohistochemistry;R language was used to analyze the expression of CAPRIN1 and PDPN mRNA in CRC adjacent tissues and cancer tissues in the Cancer Genome Atlas(TCGA)database;Pearson correlation analysis was used to study the correlation between CAPRIN1,PDPN mRNA expression and invasion and metastasis gene mRNA expression;Kaplan-Meier curve was used to analyze the effect of CAPRIN1 and PDPN protein expression on the survival prognosis of CRC patients;Cox regression analysis was used to ana-lyze the prognostic factors of CRC patients.Results The results of TCGA database analysis showed that the expression of CAPRIN1 and PDPN mRNA in CRC cancer tissues was higher than that in adjacent tissues(t/P=13.634/＜0.001,8.866/0.003).The relative expression levels of CAPRIN1,PDPN,N-cad and Vim mRNA in cancer tissues of CRC patients were higher than those in adjacent tissues,and the relative expression level of E-cad mRNA was lower than that in adjacent tissues(t/P=45.444/＜0.001,49.623/＜0.001,44.997/＜0.001,41.465/＜0.001,26.139/＜0.001).Pearson correlation analysis showed that the relative expression of CAPRIN1 and PDPN mRNA in CRC cancer tissues was positively correlated with the relative expres-sion of N-cad and Vim mRNA,and negatively correlated with the relative expression of E-cad mRNA(CAPRIN1:r/P=0.706/＜0.001,0.630/＜0.001,-0.712/＜0.001;PDPN:r/P=0.739/＜0.001,0.662/＜0.001,0.640/＜0.001).The positive rates of CAPRIN1 and PDPN protein in cancer tissues of CRC patients were 71.21％(94/132)and 74.24％(98/132),respectively,which were higher than 8.33％(11/132)and 10.61％(14/132)in adjacent tissues(x2/P=108.937/＜0.001,109.421/＜0.001).The positive rates of CAPRIN1 and PDPN proteins in CRC tissues with TNM stage Ⅲ and lymph node metastasis were high-er than those with TNM stage Ⅰ-Ⅱ and no lymph node metastasis(x2/P=10.407/0.001,7.955/0.005,10.160/0.001,7.659/0.006).The 3-year overall survival rate of CAPRIN1 positive group was 57.47％(54/94),which was lower than 73.68％(28/38)of CAPRIN1 negative group(Log-rank x2=3.821,P=0.014).The 3-year overall survival rate of PDPN positive group was 57.14％(56/98),which was lower than 76.47％(26/34)of PDPN negative group(Log-rank x2=4.154,P=0.006).Multivari-ate Cox regression analysis showed that TNM stage Ⅲ,lymph node metastasis,CAPRIN1 positive and PDPN positive were independent risk factors affecting the prognosis of CRC patients[HR(95％CI)=1.323(1.085-1.613),1.362(1.094-1.696),1.323(1.103-1.588),1.301(1.110-1.525)].Conclusion The mRNA and protein expression of CAPRIN1 and PDPN are elevated in CRC cancer tissues,which are associated with the expression of invasion and metastasis genes and serve as tumor markers for prognosis evaluation of CRC.关键词
结直肠癌/细胞周期相关蛋白1/平足蛋白/侵袭转移基因/预后Key words
Colorectal cancer/Cell cycle associated protein 1/Podoplanin/Invasion and metastasis genes/Prognosis分类
医药卫生引用本文复制引用
杨峰,王宇澄,唐煜欣,蔡永昌,吴泽建..结直肠癌组织中CAPRIN1、PDPN表达与侵袭转移基因的相关性及预后意义[J].疑难病杂志,2025,24(7):820-825,837,7.基金项目
广东省医学科学技术研究基金项目(B2019176) Guangdong Medical Science and Technology Research Fund Project(B2019176) （B2019176）
