医学信息2025，Vol.38Issue(14)：1-12,12.DOI:10.3969/j.issn.1006-1959.2025.14.001
CSF2作为肺鳞状细胞癌预后因子及相关肿瘤微环境指标的可能性研究
A Study on the Possibility of CSF2 as a Prognostic Factor and Related Tumor Microenvironmental Indicators for Lung Squamous Cell Carcinoma
摘要
Abstract
Objective To find the indicators related to the progression and prognosis of LUSC,and to explore whether colony stimulating factor 2(CSF2)is a prognostic factor and tumor microenvironment index of LUSC.Methods The gene expression profile data related to LUSC in TCGA database were downloaded.The proportion of mesenchymal components,immune cells and tumor infiltrating immune cells(TICs)in LUSC patients was calculated by estimation method and CiberSort method.Gene set enrichment analysis(GSEA)was performed on CSF2 to evaluate the proportion of TICs,and association analysis was performed to further determine the relationship between CSF2 and related clinical features of LUSC individuals.In vivo experiments,the expression of CSF2 was knocked out,and cell counting kit-8(CCK-8),reverse pore analysis,wound healing and colony formation were used to prove the proliferation,migration and invasion of LUSC cells.Results The expression of CSF2 in tumor tissues was significantly higher than that in normal tissues,and CSF2 overexpression was associated with lower survival rate of LUSC patients(P＜0.05).GO and KEGG analysis showed that CSF2 might be considered as a potential indicator of tumor microenvironment status(TME).In addition,CSF2 knockdown led to a significant decrease in cell proliferation,migration and invasion(P＜0.05).Conclusion CSF2 may be a potential therapeutic target and prognostic indicator for TME in LUSC patients.关键词
肺鳞状细胞癌/肿瘤微环境/集落刺激因子2/临床病理和预后Key words
Lung squamous cell carcinoma/Tumor microenvironment/Colony stimulating factor 2/Clinical pathology and prognosis分类
医药卫生引用本文复制引用
贺先明,姜斯聪,邢雨轩,曾雷..CSF2作为肺鳞状细胞癌预后因子及相关肿瘤微环境指标的可能性研究[J].医学信息,2025,38(14):1-12,12.基金项目
1.江西省自然科学基金(编号:20202BAB206057) （编号:20202BAB206057）
2.江西省应用研究培育项目(编号:20212BAG70047) （编号:20212BAG70047）
3.北京希思科临床肿瘤学研究基金会资助项目(编号:Y-XD202001/zb-0002) （编号:Y-XD202001/zb-0002）
4.南昌大学第二附属医院院内资助项目(编号:2022efyB05) （编号:2022efyB05）
