园艺学报2025，Vol.52Issue(6)：1399-1411,13.DOI:10.16420/j.issn.0513-353x.2024-0478
枳PtSARD1的特征分析及其抗旱功能初探
Characterization of PtSARD1 and Its Preliminary Role in Drought Tolerance
摘要
Abstract
Systemic Acquired Resistance Deficient 1(SARD1)is a transcription factor related to salicylic acid synthesis.In order to investigate its role in drought response,the 1 425 bp open reading frame of SARD1 in Poncirus trifoliata(named as PtSARD1)was obtained.The characterization of PtSARD1 was preliminarily studied by sequence analysis,subcellular localization,relative expression level,overexpression(OE),RNA interference(RNAi)and tolerance-related physiological indicators measurement.The results revealed that PtSARD1 contained three calmodulin binding protein domains and the subcellular localization of this protein will change over time,transferring from the nucleus to the cell membrane.The expression of PtSARD1 was detected in root,stem and leaf,with higher expression in leaf.Meanwhile,PtSARD1 was induced by drought and exogenous application of salicylic acid.PtSARD1-RNAi P.trifoliata and PtSARD1-OE tobacco were obtained to study its function in drought response.The detached leaves of two PtSARD1-RNAi P.trifoliata lines showed higher relative water loss at each time point.Relative water loss in detached leaves of two PtSARD1-OE tobacco lines(A7 and F7)were lower than that of the wild type tobacco(WT).When water was withheld,potted seedlings of A7 and F7 showed better growth status,higher proline content,but lower electrolyte leakage and malondialdehyde content.Overall,these results indicated that PtSARD1 may play a positive regulatory role in the drought response of P.trifoliata.关键词
柑橘/抗旱/基因功能/水杨酸/SARD1Key words
citrus/drought tolerance/gene function/salicylic acid/SARD1分类
农业科技引用本文复制引用
莫锦夏,李芳,熊欣婷,钟灶发,彭婷..枳PtSARD1的特征分析及其抗旱功能初探[J].园艺学报,2025,52(6):1399-1411,13.基金项目
江西省自然科学基金项目(20212ACB205001) （20212ACB205001）
国家自然科学基金项目(32260749) （32260749）
