园艺学报2025，Vol.52Issue(6)：1412-1426,15.DOI:10.16420/j.issn.0513-353x.2024-0603
转录—代谢联合分析'贵妃'杧果腋芽转化花芽中的淀粉与蔗糖代谢途径
Joint Transcription-Metabolism Analysis of Starch and Sucrose Metabolic Pathways in the Transformation of Axillary Buds into Flower Buds of 'Guifei' Mango
摘要
Abstract
Removal of apical inflorescences in mango can promote the transformation of axillary buds to flower buds and delay the flowering time of plants,thus avoiding the damage of"late spring coldness".Transcriptomic and metabolomic data were measured during the differentiation of axillary buds into flower buds after removal of apical flower buds in 'Guifei' mango,and differentially expressed genes and differential metabolites of starch and sucrose metabolism pathways during axillary bud development were jointly analyzed to clarify their temporal and spatial dynamic changes and expression patterns.The results showed that a total of 76 differentially expressed genes related to starch and sucrose metabolism,involving 17 enzymes,and five differential metabolites were identified during the transformation of axillary buds into flower buds;the expression of SUS(LOC123200616),FRK(LOC123221640),and TPP(LOC123202828)was up-regulated after the apical flower buds were removed;the expression of AMY(LOC123229947)was down-regulated,and the expression trends of the four genes were consistent with the transcriptome analysis.The above results suggested that flower bud differentiation is species-biased for the conversion and utilization of sugars,and mainly relies on the conversion and utilization of sucrose and glucose-6-phosphate;D-fructose and α,α-Trehalose can induce flower formation at specific periods.It is suggested that controlling the ratio of different sugars in axillary buds is beneficial to regulate the flowering period of mango.关键词
杧果/腋芽/淀粉与蔗糖代谢途径/转录组/代谢组Key words
mango/axillary bud/starch and sucrose metabolism pathway/transcriptome/metabolome分类
农业科技引用本文复制引用
杨春梅,于洋,丁雨格,夏京,周玲,彭磊..转录—代谢联合分析'贵妃'杧果腋芽转化花芽中的淀粉与蔗糖代谢途径[J].园艺学报,2025,52(6):1412-1426,15.基金项目
云南省科技厅乡村振兴专项 （）
创新引导与科技型企业培育计划(202404BI090012) （202404BI090012）
