园艺学报2025，Vol.52Issue(6)：1451-1462,12.DOI:10.16420/j.issn.0513-353x.2024-0929
白色桑椹颜色缺失分子机制初步探讨
Preliminary Research on the Molecular Mechanism of Color Loss in White Mulberry Fruit
摘要
Abstract
In order to reveal the mechanism of anthocyanin synthesis blockade in white mulberry,the metabolome and transcriptome of white mulberry and black mulberry were compared and analyzed.The results showed that the anthocyanin content of 'Anshen'(black fruit)gradually accumulated during the ripening process,and the accumulation amount was the highest in the ripening stage,while the anthocyanin content of 'Guihua'(white fruit)was the highest in the green fruit stage,and the anthocyanin content was lower at the veraison and maturity stages.The key color substances of anthocyanin glycosides,such as cyanidin,delphinin,pelargondin,malvidin pigment and petunidin,all had low accumulation in 'Guihua',especially cyanidin-3-O-glucoside,cyanidin-3-O-rutinoside,delphin-3-O-glucoside,delphin-3-O-loccoside,pelargondin-3-O-glucoside,pelargondin-3-O-rutinoside,proanthocyanidin B1,proanthocyanidinin B3 and other substances showed a large accumulation in 'Anshen'.The expression of PAL,C4H,4CL,CHS,CHI,F3H and other genes involved in the biosynthesis of anthocyanins in 'Guihua' decreased during veraison,and this down-regulation prevented the accumulation of anthocyanins in white mulberry.Based on transcriptome data,six differentially expressed MYB transcription factor genes were identified,and combined with gene expression,evolutionary analysis and downstream target gene identification,it was analyzed that MYB6 may play an inhibitory role in the synthesis of anthocyanin glycosides during the maturation of white mulberry.关键词
桑椹/转录组/代谢组/花青素/结构基因/MYB6Key words
mulberry/transcriptome/metabolome/anthocyanin/structural gene/MYB6分类
农业科技引用本文复制引用
郭新淼,田露瑶,曹佳琪,谢岩,高妍夏,孙志超..白色桑椹颜色缺失分子机制初步探讨[J].园艺学报,2025,52(6):1451-1462,12.基金项目
河北省自然科学基金项目(C2019406113和H2023406059) （C2019406113和H2023406059）
承德市基础研究专项(202205B069) （202205B069）
河北省'三三三人才工程'资助项目(A202101051) （A202101051）
河北省教育厅一般项目(QN2020236) （QN2020236）
