园艺学报2025，Vol.52Issue(6)：1463-1476,14.DOI:10.16420/j.issn.0513-353x.2024-0160
黄瓜脱镁螯合酶基因CsMDC表达分析及其调控叶绿素降解的功能验证
Expression Analysis of the Cucumber Mg-Dechelatase Gene CsMDC and Functional Verification of Its Role in Regulating Chlorlorophyll Degradation
摘要
Abstract
In this study,cucumber cultivar 'Zhongnong 12'(Cucumis sativus L.)was used to analyze the expression of CsMDC in cucumber and further verify its role in the process of chlorophyll degradation.The analysis and prediction of the physicochemical properties of the CsMDC protein revealed that it has the highest similarity to melon(Cucumis melo L.)from the same family and genus,followed by plants from the same family but different genera such as wax gourd(Benincasa hispida Cogn.),pumpkin(Cucurbita moschata Duch.),and squash(Cucurbita maxima Duch.).Its similarity to plants from other families is relatively low,indicating that MDC has distinct species-specific characteristics.qRT-PCR analysis of the expression patterns of CsMDC under different exogenous growth regulator treatments and abiotic stresses showed that the gene has the highest relative expression in senescent tissues and exhibits different response characteristics to various conditions.Functional analysis through the construction of CsMDC-overexpressing tobacco plants demonstrated that the overexpression of CsMDC resulted in significantly lower chlorophyll content,dry-to-fresh weight ratio,and net photosynthetic rate compared to wild-type plants,while the reactive oxygen species content was significantly higher.qRT-PCR results showed that the expression of photosystem protein genes in the CsMDC-overexpressing tobacco plants significantly decreased after dark stress,while the expression of genes related to chlorophyll degradation significantly increased.These findings suggest that CsMDC in cucumber is closely related to chlorophyll degradation,induced by different hormones and environmental stresses,and positively regulates chlorophyll degradation and plant senescence.关键词
黄瓜/脱镁螯合酶/CsMDC/表达模式/叶绿素降解/衰老/非生物胁迫Key words
cucumber/Mg-dechelatase/CsMDC/expression pattern/chlorophyll degradation/senescence/abiotic stress分类
农业科技引用本文复制引用
李媚晴,罗思菲,贾曜豪,王维贵,孙锦..黄瓜脱镁螯合酶基因CsMDC表达分析及其调控叶绿素降解的功能验证[J].园艺学报,2025,52(6):1463-1476,14.基金项目
国家自然科学基金项目(31872152) （31872152）
现代农业产业技术体系建设专项资助(CARS-23) （CARS-23）
