园艺学报2025，Vol.52Issue(6)：1477-1487,11.DOI:10.16420/j.issn.0513-353x.2024-0084
番茄抗青枯病种质TK083的筛选和鉴定
Screening and Identification for Bacterial Wilt Resistance Accession TK083 in Tomato
摘要
Abstract
In the northern part of Jiangxi Province,130 tomato accessions were naturally screened for bacterial wilt resistance,and the seedling resistance of these accessions to the local dominant Ralstonia solanacearum strain RS 100 was verified,and TK083 was found to be an excellent tomato resistant to bacterial wilt accessions.After inoculation with Ralstonia solanacearum,the concentration of the pathogen in the xylem sap of TK083 was much lower than that of susceptible tomato 'Moneymaker'.The activities of SOD and POD in the leaves of TK083 increased markedly,while the MDA content showed a slight increase in the early stages post-inoculation.The activity of chitinase and β-1,3-GA increased in the early and late stages of inoculation respectively,indicating that they were involved in different stages of immune response.RT-qPCR results revealed that the expression levels of SlNAP1,SlERF2a and SlERF3,which are involved in the immune regulation of bacterial wilt,were significantly up-regulated by Ralstonia solanacearum in TK083 roots.These results suggested that TK083 likely employs multiple pathways to coordinately combat the infection of Ralstonia solanacearum.关键词
番茄/青枯病/青枯雷尔氏菌/种质/抗性/鉴定Key words
tomato/bacterial wilt/Ralstonia solanacearum/accession/resistance/identification分类
农业科技引用本文复制引用
方俊仪,巫伟峰,陆乔,凌宏清,孔丹宇..番茄抗青枯病种质TK083的筛选和鉴定[J].园艺学报,2025,52(6):1477-1487,11.基金项目
国家自然科学基金地区基金项目(32260768) （32260768）
江西省人才项目(jxsq2020101088) （jxsq2020101088）
九江市人才项目(jjsbsq2020005) （jjsbsq2020005）
九江市基础研究计划(S2024KXJJ0001) （S2024KXJJ0001）
