针刺研究2025，Vol.50Issue(7)：735-742,8.DOI:10.13702/j.1000-0607.20241267
从脊髓背角α7烟碱乙酰胆碱受体调控神经炎性反应探讨腕踝针治疗化疗性神经痛小鼠的作用机制
Exploring the mechanism of wrist-ankle acupuncture in treating chemotherapy-induced neuropathic pain in mice by regulating neuroinflammatory responses through α7 nicotinic acetylcholine receptor in the spinal dorsal horn
摘要
Abstract
Objective To investigate the effect of wrist-ankle acupuncture on the α7 nicotinic acetylcholine receptor(α7nAChR)/Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway in the spinal dorsal horn of mice with chemotherapy-induced neuropathic pain(CINP),and to explore the mechanisms by which low-intensity wrist-ankle electroacupuncture alleviates pain hypersensitivity in CINP mice.Methods C57BL/6 mice were randomly divided into a blank group,a model group,a wrist-ankle acupuncture group,and a wrist-ankle acupuncture+antagonist group,with 8 mice in each group.A CINP model was established by intraperitoneal injection of paclitaxel(2 mg/kg).The wrist-ankle acupuncture group received electroacupuncture at the"Lower 4"and"Lower 5"points in the wrist-ankle region for 30 min per session,once every other day,for a total of 7 sessions.The wrist-ankle acupuncture+antagonist group received intrathecal injections of the α7nAChR antagonist α-BGT(0.5 μg/kg)on day 1,7,and 13,in addition to acupuncture.The mechanical and thermal pain thresholds were assessed using Von Frey filaments and hot plate tests.The expression level of α7nAChR protein in the spinal dorsal horn(L4-L6)was determined by Western blot.Immunofluorescence staining was used to detect the expression of ionized calcium-binding adapter molecule 1(Iba-1)in the spinal dorsal horn.ELISA was used to measure the relative levels of phosphorylated(p)-JAK2 and p-STAT3 in the spinal dorsal horn.Quantitative PCR was used to detect the relative mRNA expression levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6).Results Compared with the blank group,the model group exhibited decreased mechanical and thermal pain thresholds(P<0.001),reduced α7nAChR protein expression(P<0.05),increased Iba-1 expression(P<0.05),elevated relative contents of p-JAK2 and p-STAT3(P<0.05),and increased mRNA expression levels of TNF-α,IL-1β,and IL-6(P<0.05).Compared with the model group,the wrist-ankle acupuncture group showed increased mechanical and thermal pain thresholds(P<0.05),elevated α7nAChR protein expression(P<0.05),decreased Iba-1 expression(P<0.05),reduced relative contents of p-JAK2 and p-STAT3(P<0.05),and decreased mRNA expression levels of TNF-α,IL-1β,and IL-6(P<0.05).Compared with the wrist-ankle acupuncture group,the wrist-ankle acupuncture+antagonist group showed decreased mechanical and thermal pain thresholds(P<0.05),lower α7nAChR protein expression(P<0.05),increased Iba-1 expression(P<0.05),higher relative contents of p-JAK2 and p-STAT3(P<0.05),and elevated mRNA expression levels of TNF-α,IL-1β,and IL-6(P<0.05).Conclusion Wrist-ankle acupuncture may alleviate pain hypersensitivity in CINP mice by regulating the α7nAChR/JAK2/STAT3 signaling pathway in the spinal dorsal horn,inhibiting microglial activation,and reducing neuroinflammation.关键词
腕踝针/电针/化疗性神经痛/α7烟碱乙酰胆碱受体/Janus激酶/信号转导因子和转录激活因子3信号通路Key words
Wrist-ankle acupuncture/Electroacupuncture/Chemotherapy-induced neuropathic pain/α7 nicotinic acetylcholine receptor/Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway引用本文复制引用
陈小梅,庞莉娜,柯以晨,黄秋玲,兰艳艳,俞向梅,叶晓倩,王志福..从脊髓背角α7烟碱乙酰胆碱受体调控神经炎性反应探讨腕踝针治疗化疗性神经痛小鼠的作用机制[J].针刺研究,2025,50(7):735-742,8.基金项目
国家自然科学基金面上项目(No.82274658) （No.82274658）
福建省自然科学基金项目(No.2022J01347、2023J01333) （No.2022J01347、2023J01333）
福建省卫健委医学创新课题(No.2022CXA053) （No.2022CXA053）
福建中医药大学针灸学科开放课题(No.XZJ2024002) （No.XZJ2024002）
福建省中青年教师教育科研项目(No.JAT220116) （No.JAT220116）
"十四五"国家中医药管理局中医康复学科开放课题(No.X2023010) （No.X2023010）
福建中医药大学校管课题(No.X2023014) （No.X2023014）
