针刺研究2025，Vol.50Issue(7)：799-807,9.DOI:10.13702/j.1000-0607.20240421
经筋刺法对膝关节骨关节炎大鼠骨骼肌卫星细胞增殖分化的影响
Effect of Jingjin needling on the proliferation and differentiation of skeletal muscle satellite cells in rats with knee osteoarthritis
摘要
Abstract
Objective To observe the effects of the Jingjin(meridian-muscle region)needling on muscle growth markers,inflammatory factors,and proteins of Wnt/β-catenin signaling pathway in skeletal muscle of rats with knee osteoarthritis(KOA),so as to explore its possible mechanisms in treating KOA.Methods Male SD rats were randomly divided into three groups:blank group,model group,and Jingjin needling group,with 10 rats in each group.The KOA model was established using a knee joint immobilization method.In the Jingjin needling group,acupuncture was applied to the tendon insertion points of the rectus femoris muscle at the anterior thigh,the tendon insertion point of the rectus femoris muscle in the groin area,and spastic nodule of the soft tissue between the semitendinosus and biceps femoris long head at the ischial tuberosity on the right side,for 20 minutes per session,once a day,for a total of 14 days.Gait analysis technology was used to observe the effects of the acupuncture method on the behavior of rats.HE staining was used to observe the morphological structure of skeletal muscle.ELISA was used to detect the contents of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in skeletal muscle.Real time quantitative fluorescent PCR was utilized to measure mRNA expression levels of paired box protein 7(Pax7),myogenic differentiation antigen(MyoD),myogenin(MyoG),and myosin heavy chain-Ⅰ(MyHC1)in skeletal muscle.Western blot was used to assess the protein expression levels of β-catenin,glycogen synthase kinase 3β(GSK3β),phosphorylated β-catenin(p-β-catenin),and G1/S-specific cyclin D1(CyclinD1)in skeletal muscle.Results The model group exhibited damaged muscle fibers with noticeable disarray and infiltration of inflammatory cells;in contrast,the Jingjin needling group showed relatively intact muscle fiber structures and more orderly fiber arrangements,along with newly regenerated fibers.After acupuncture intervention,compared with the blank group,the model group showed decreased paw print area,maximum pressure,muscle wet weight,wet weight ratio,and hind limb circumference(P<0.01,P<0.05);and increased contents of IL-1β and TNF-α in skeletal muscle(P<0.01);and elevated mRNA expression of Pax7,MyoD,and MyoG(P<0.01),while MyHC1 mRNA expression decreased(P<0.01).The protein expressions of β-catenin and CyclinD1 in skeletal muscle were increased(P<0.05,P<0.01),while those of GSK3β and p-β-catenin were decreased(P<0.05,P<0.01).Compared to the model group,the Jingjin needling group rats demonstrated increased paw print area,maximum pressure,muscle wet weight,wet weight ratio,and hind limb circumference(P<0.01);reduced contents of IL-1β and TNF-α in skeletal muscle(P<0.01,P<0.05);and elevated mRNA expressions of Pax7,MyoD,MyoG,and MyHC1(P<0.01).Moreover,the protein expressions of β-catenin and CyclinD1 in skeletal muscle were increased(P<0.01),while those of GSK3β and p-β-catenin were decreased(P<0.01).Conclusion Jingjin needling can promote the proliferation and differentiation of skeletal muscle satellite cells in KOA rats and exert regenerative and reparative effects by up-regulating the Wnt/β-catenin signaling pathway.关键词
膝关节骨关节炎/经筋/针刺/卫星细胞/生肌标志物/炎性因子/β-连环蛋白Key words
Knee osteoarthritis/Jingjin/Acupuncture/Satellite cells/Muscle growth markers/Inflammatory factors/β-catenin引用本文复制引用
富昱,董宝强,林星星,盛泽伟,田昀昊,王垒钞..经筋刺法对膝关节骨关节炎大鼠骨骼肌卫星细胞增殖分化的影响[J].针刺研究,2025,50(7):799-807,9.基金项目
国家自然科学基金项目(No.81574050) （No.81574050）
辽宁省自然科学基金计划项目(No.2022-BS-196、2022-NLTS-13-04) （No.2022-BS-196、2022-NLTS-13-04）
