诊断学理论与实践2025，Vol.24Issue(1)：35-42,8.DOI:10.16150/j.1671-2870.2025.01.006
外周血Scimp可作为代谢相关脂肪性肝病肝硬化的生物诊断标志物——基于动物实验的研究
Study on exploring Scimp in peripheral blood as a biodiagnostic marker for MAFLD-related cirrhosis based on animal experiments
摘要
Abstract
Objective This study aims to identify biodiagnostic markers for the progression of metabolic-associated fatty liver disease(MAFLD)to cirrhosis,providing evidence for early diagnosis and treatment of cirrhosis.Methods The experiment utilized healthy male Sprague Dawley(SD)rats,which were randomly divided into a normal control(NC)group of 15 rats,a high-fat diet(HFD)group of 15 rats,and a liver fibrosis(LF)group of 15 rats.Rats in the NC group were fed a normal diet,those in the HFD group received continuous high-fat diet to induce fatty liver and mild liver fibrosis,and those in the LF group were fed a high-fat diet and injected with carbon tetrachloride to induce cirrhosis.After 41 weeks of feeding,blood and liver tissues were collected from rats.Liver tissues were stained with hematoxylin and eosin(HE),Oil Red O,and Sirius red to evaluate liver morphology,fat infiltration,and liver fibrosis.Differentially expressed genes in blood samples were screened using mRNA sequencing technology,and the expression of sequencing-screened differen-tially expressed genes was validated by reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR).Additionally,blood samples from 10 human subjects each in the NC,MAFLD,and cirrhosis groups were collected from January to February 2025 at Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine to validate the mRNA expression of screened differentially expressed genes.Results The mRNA sequencing of peripheral blood cells in rats identified 17 differentially expressed genes,including Rab3ip,Gprasp2,Emid1,Hbq1b,Scimp,Wipf3,Scrn1,Sez6,Bglap,Bhlhb9,Ranbp10,Ubd,Plekhb1,Nup210l,Gp1bb,Cpne8,and Oscar.The RT-qPCR validation results showed that the mRNA expression levels of the Scimp gene in the NC group,HFD group,and LF group exhibited an increasing trend in sequence,which was consistent with the sequencing results.Pairwise comparisons between groups showed statistically sig-nificant differences(P<0.01).The RT-qPCR validation results of human peripheral blood cells were consistent with the mRNA sequencing and RT-qPCR validation expression trends of the Scimp gene in rat peripheral blood.Scimp showed the same expression differences among NC,MAFLD,and cirrhosis patients(P<0.05).Conclusions The mRNA expression level of the Scimp gene in peripheral blood cells can serve as a potential non-invasive biodiagnostic marker for early-stage cirrhosis.关键词
代谢相关脂肪性肝病/肝硬化/诊断标志物/Scimp基因Key words
Metabolic-associated fatty liver disease/Cirrhosis/Biodiagnostic marker/Scimp gene分类
医药卫生引用本文复制引用
陈洪卫,朱婷,刘燕,侯彦强,范广建..外周血Scimp可作为代谢相关脂肪性肝病肝硬化的生物诊断标志物——基于动物实验的研究[J].诊断学理论与实践,2025,24(1):35-42,8.基金项目
上海市松江区科学技术攻关项目(22SJKJGG73) （22SJKJGG73）
上海市松江区公共卫生体系建设三年行动计划项目(SJGW6-28) （SJGW6-28）
上海市医学重点学科(2024ZDXK0065) （2024ZDXK0065）
