中风与神经疾病杂志2025，Vol.42Issue(7)：637-640,4.DOI:10.19845/j.cnki.zfysjjbzz.2025.0122
脑卒中GSK-3β调控内质网-线粒体偶联参与炎症性脑损伤的机制研究
Mechanism of glycogen synthase kinase-3β participating in stroke-induced inflammatory brain injury by regulating endoplasmic reticulum-mitochondria coupling
摘要
Abstract
Objective To investigate the mechanism of glycogen synthase kinase-3β(GSK-3β)participating in stroke-induced inflammatory brain injury through endoplasmic reticulum-mitochondria coupling,and to provide a theoreti-cal reference and potential targets for precise treatment.Methods A rat model of middle cerebral artery occlusion was es-tablished,and the rats were divided into sham-operation group,model group,and GSK-3β inhibitor(TDZD-8)intervention group.Neurological function score was used to evaluate the degree of brain injury;TTC staining was used to evaluate cere-bral infarct area;electron microscopy was used to observe mitochondrial ultrastructure;mitochondrial calcium concentra-tion was measured;ELISA was used to measure the levels of inflammatory factors[tumor necrosis factor-α(TNF-α)and in-terleukin-1β(IL-1β)],and Western blot was used to measure the expression levels of related proteins.Results Compared with the sham-operation group,the model group had significant neurological function impairment,an increase in cerebral infarct area,and significant increases in the expression levels of inflammatory factors and GSK-3β,with enhanced endoplas-mic reticulum-mitochondria coupling and mitochondrial calcium overload.TDZD-8 significantly improved the above indica-tors and reduced the levels of inflammatory factors and cerebral infarct area.Conclusion Abnormal activation of GSK-3β damages brain tissue by regulating endoplasmic reticulum-mitochondria coupling and promotes mitochondrial calcium over-load and inflammatory response.Inhibiting GSK-3β activity can effectively improve stroke-induced inflammatory brain in-jury,which provides a new pathway and a molecular target for developing precise treatment strategies.关键词
脑卒中/糖原合成激酶-3β/内质网-线粒体偶联/脑损伤Key words
Stroke/Glycogen synthase kinase-3β/Endoplasmic reticulum-mitochondria coupling/Brain injury分类
医药卫生引用本文复制引用
雷建华,杜贺芳,刘昱肖..脑卒中GSK-3β调控内质网-线粒体偶联参与炎症性脑损伤的机制研究[J].中风与神经疾病杂志,2025,42(7):637-640,4.基金项目
2024河北省医学科学研究课题计划项目(20241660) （20241660）
