中国病理生理杂志2025，Vol.41Issue(7)：1267-1274,8.DOI:10.3969/j.issn.1000-4718.2025.07.003
黄芪-当归合剂对脑缺血再灌注大鼠神经细胞焦亡的影响
Effect of Huangqi-Danggui mixture on neural cell pyroptosis in cerebral ischemia/reperfusion rats
摘要
Abstract
AIM:To observe the effects of Huangqi-Danggui mixture(HQDG)on the pyroptosis of brain tis-sues in rats with middle cerebral artery occlusion/reperfusion(MCAO/R),and to explore the mechanism of neuroprotec-tion provided by HQDG.METHODS:Sixty-four SD rats were randomly divided into 4 groups:sham group,model group,HQDG group,and Xuesaitong(XST)group.The infarct volume of brain tissues was observed by 2,3,5-triphenyl-tetrazolium chloride staining,while hematoxylin-eosin staining was used to observe the pathological changes of brain tis-sues.Immunofluorescence was employed to assess the expression of nucleotide-binding oligomerization domain-like recep-tor protein 3(NLRP3),cleaved caspase-1 and gasdermin D(GSDMD)in the ischemic penumbra of brain tissues.The se-rum levels of interleukin-1β(IL-1β)and IL-18 were measured using ELISA.Western blot was used to detect NLRP3,cleaved caspase-1,apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),IL-1β and IL-18 in brain tissues.RESULTS:Compared with sham group,the neurological deficit scores of the rats in model group were significantly increased(P<0.01),while those in HQDG and XST groups were significantly reduced compared with model group(P<0.01).The cerebral infarct volume ratio was significantly reduced in HQDG and XST groups compared with model group(P<0.01).The pathological damage of brain tissue in HQDG and XST groups was significantly reduced compared with model group.The positive rates of NLRP3,cleaved caspase-1 and GSDMD in the ischemic penumbra of brain tissues were significantly decreased in HQDG group compared with model group(P<0.01).The expression of pyrop-tosis-related proteins,NLRP3,cleaved caspase-1 and ASC,in the ischemic penumbra of brain tissues was significantly el-evated in model group compared with sham group(P<0.01),and significantly decreased in HQDG group compared with model group(P<0.01).The serum levels of IL-1β and IL-18 were significantly increased in model group compared with sham group(P<0.01),and significantly reduced in HQDG group compared with model group(P<0.01).CONCLU-SION:The HQDG effectively attenuates brain tissue injury in rats with MCAO/R,and its mechanism may be related to the inhibition of neural cell pyroptosis.关键词
黄芪-当归合剂/脑缺血再灌注损伤/焦亡/神经保护Key words
Huangqi-Danggui mixture/cerebral ischemia-reperfusion injury/pyroptosis/neuroprotection分类
医药卫生引用本文复制引用
王瑞坤,高维娟,侯仙明,邢志峰,刘璐瑶,柴成萱,张怡..黄芪-当归合剂对脑缺血再灌注大鼠神经细胞焦亡的影响[J].中国病理生理杂志,2025,41(7):1267-1274,8.基金项目
国家自然科学基金青年基金项目(No.82104963) （No.82104963）
河北省中医药管理局科研计划项目(No.2024336) （No.2024336）
河北中医药大学燕赵医学研究项目(No.YZZY2023001 （）
No.YZZZ2022002) （）
河北省高等学校科学技术研究项目(No.QN2022069) （No.QN2022069）
