中国病理生理杂志2025，Vol.41Issue(7)：1317-1324,8.DOI:10.3969/j.issn.1000-4718.2025.07.008
一种特定合生元对ApcMin/+小鼠结肠肿瘤发生的抑制作用
Inhibitory effect of a specific synbiotic on colon tumorigenesis in ApcMin/+mice
摘要
Abstract
AIM:This study aims to investigate whether a specific synbiotic,consisting of Bifidobacterium longum subspecies BB536 and fructooligosaccharides,can inhibit colon tumorigenesis by modulating gut flora composi-tion.METHODS:Six-week-old male ApcMin/+mice were randomly divided into control group and synbiotic-treated group,with 8 mice in each group.The mice in synbiotic-treated group received daily gavage of 1×109 CFU/kg of Bifidobacterium longum BB536 and 2 g/kg of fructooligosaccharides,while those in control group were gavaged with sterile water for 10 weeks.The number and size of colon tumors were assessed and compared between the 2 groups.Colon tissue sections were evaluated using hematoxylin-eosin staining,Alcian blue staining,and immunohistochemistry assays.The bacterial compo-sition in the colon was analyzed via 16S rDNA sequencing,and differentially expressed genes were examined through tran-scriptome sequencing and RT-qPCR.RESULTS:The synbiotic treatment significantly enhanced bacterial diversity and increased the relative abundance of Bifidobacterium in the colon(P<0.01).Additionally,the number and size of colon tu-mors were both significantly reduced in the synbiotic-treated mice(P<0.01),along with decreased expression of Ki-67(P<0.01).Concurrently,the number of goblet cells and the expression of epithelial tight junction proteins(ZO-1 and occlu-din)were up-regulated(P<0.05).CONCLUSION:Supplementation with this synbiotic effectively modulated gut flora composition and suppressed colon tumorigenesis in ApcMin/+mice.关键词
结直肠癌/肠道菌群/益生菌/益生元/合生元Key words
colorectal cancer/gut flora/probiotic/prebiotic/synbiotic分类
医药卫生引用本文复制引用
郑姣,吴辉霞,仝谣,文泓,刘如石,廖旻晶..一种特定合生元对ApcMin/+小鼠结肠肿瘤发生的抑制作用[J].中国病理生理杂志,2025,41(7):1317-1324,8.基金项目
湖南省重点研发计划项目(No.2019SK2042) （No.2019SK2042）
湖南省自然科学基金面上项目(No.2023JJ30428) （No.2023JJ30428）
湖南省卫生健康委科研计划课题(No.D202302089038) （No.D202302089038）
2022年度湖南省大学生创新创业训练计划项目(No.S202210542145) （No.S202210542145）
湖南师范大学与韶山常佰通生物科技有限公司产学研合作项目(No.201808003) （No.201808003）
