中国草地学报2025，Vol.47Issue(7)：1-12,12.DOI:10.16742/j.zgcdxb.20240468
花苜蓿Dof家族基因鉴定及其在干旱胁迫下的表达模式分析
Identification of the Dof Family Gene in Medicago ruthenica and Expression Analysis Pattern under Drought Stress
摘要
Abstract
Dof(DNA-binding with one finger)transcription factors are plant-specific regulators play-ing critical roles in plant response to environmental stresses.This study comprehensively identified MrDof gene family based on the genome-wide of Medicago ruthenica.We performed analyses including collinear-ity assessment,phylogenetic analysis,and chromosomal localization on the Dof family members.Further-more,by integrating transcriptome data of M.ruthenica under drought stress and qRT-PCR validation,we explored the potential functions of these genes in drought stress responses.The results showed that a total of 33 MrDof genes were identified in M.ruthenica,and the number of amino acids encoded by these genes ranged from 157 to 492 aa.According to the evolutionary relationships among M.ruthenica,Medi-cago truncatula and Arabidopsis thaliana,these genes were classified into eight distinct groups(Group A,B1,B2,C1,C2.1,C2.2,C3,D1 and D2).The 33 genes were unevenly distributed across nine chromo-somes.Expression pattern analysis demonstrated that five genes(MrDof11,MrDof13,MrDof22,MrDof27,and MrDof30)were significantly induced by drought stress.关键词
花苜蓿/Dof基因家族/干旱胁迫/系统发育分析/基因表达分析Key words
Medicago ruthenica/Dof gene family/Drought stress/Phylogenetic analysis/Gene expression analysis分类
农业科技引用本文复制引用
袁小淞,隋超,罗栋,王召明,刘志鹏,闫龙凤..花苜蓿Dof家族基因鉴定及其在干旱胁迫下的表达模式分析[J].中国草地学报,2025,47(7):1-12,12.基金项目
内蒙古自治区揭榜挂帅项目(2022JBGS0040) （2022JBGS0040）
2023年国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX20-23N04) （筹）
甘肃省首席科学家负责制项目(23ZDKA013) （23ZDKA013）
