中国草地学报2025，Vol.47Issue(7)：13-20,8.DOI:10.16742/j.zgcdxb.20240460
农杆菌介导的梭梭种子遗传转化体系初探
Preliminary Study on Agrobacterium-Mediated Genetic Transformation System of Haloxylon ammodendron Seeds
摘要
Abstract
Effects of acetosyringone(AS),cefotaxime(Cef),and the herbicide phosphinothricin(PPT)on genetic transformation of Haloxylon ammodendron seeds were explored after optimizing of Agrobacterium species,concentration,and infection time.The results showed that the Agrobacterium tumefaciens GV3101 was the optimal strain for H.ammodendron seed infection.H.ammodendron seeds with artificial wounds were infected in Agrobacterium suspension(OD600=0.6)for 10 min.After co-cultivation on the medium(MS+150 µmol/L AS)for 2 d,the seeds were transferred to the delayed selection medium containing cefotaxime(MS+250 mg/L Cef)for 10-14 d.Final selection was conducted on medium containing phosphinothricin(MS+250 mg/L Cef+1.0 mg/L PPT)for 21-28 d.H.ammodendron seedlings with PPT resistance were obtained.Plants with transgenic-positive were identified by PCR detection and GUS staining,with a transfor-mation rate ranged from 1.6%to 5.2%.关键词
梭梭种子/农杆菌介导/转化效率Key words
Haloxylon ammodendron seeds/Agrobacterium-mediated/Transformation efficiency分类
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娜菲莎·木则帕尔,孙朗,王萍,任燕萍,马丽,程聪,姚正培,王波,张桦..农杆菌介导的梭梭种子遗传转化体系初探[J].中国草地学报,2025,47(7):13-20,8.基金项目
新疆维吾尔自治区自然科学基金重点项目(2022D01D35) （2022D01D35）
