中国草地学报2025，Vol.47Issue(7)：48-60,13.DOI:10.16742/j.zgcdxb.20240434
2,4-表油菜素内酯对干旱胁迫下夏枯草种子萌发与幼苗生理特性的影响
Effects of 2,4-Epibrassinolide on Seed Germination and Seedling Physiological Characteristics of Prunella vulgaris under Drought Stress
摘要
Abstract
This study investigated the effects of exogenous 2,4-epibrassinolide(EBR)on seed germi-nation and seedling physiological characteristics of Prunella vulgaris under drought stress,to provide a theoretical basis for its efficient cultivation in arid regions.Seeds of P.vulgaris were soaked with EBR solutions at different concentrations(0,0.01,0.05,0.10,and 0.20 μmol/L),and then germinated under drought simulation by 20%polyethylene glycol(PEG-6000)to investigate effects of EBR on seed germina-tion.The results indicated that drought stress significantly inhibited seed germination and seedling growth of P.vulgaris.Meanwhile,antioxidant enzyme activities and the content of osmotic regulatory substances were increased by drought stress,with electrolyte leakage in seedlings and a decrease in photosynthetic pig-ment content.After the treatment with 0.01-0.20 μmol/L EBR,seed germination and seedling growth under drought stress were improved.EBR treatment further enhanced antioxidant enzyme activities,soluble protein content,and soluble sugar content in seedlings,while reducing relative electrical conductivity,and promoting photosynthetic pigment accumulation.When the EBR soaking concentration increased from 0.01 to 0.20 μmol/L,the alleviation effect initially intensified and then declined.The comprehensive evaluation revealed that the effects of different concentrations of EBR on the seed germination and seedling growth of Prunella vulgaris under drought stress are followed the order of 0.10 μmol/L treatment>0.05 μmol/L treat-ment>0.01 μmol/L treatment>0.20 μmol/L treatment,with the 0.10 μmol/L treatment being the most effective.关键词
夏枯草/2,4-表油菜素内酯/干旱胁迫/种子萌发/生理指标Key words
Prunella vulgaris/2,4-Epibrassinolide/Drought stress/Seed germination/Physiological parameters分类
农业科技引用本文复制引用
姚海瑞,陈轲,张利霞,谢梦圆,董和禹,宋文栩,康雅,常青山,陈根强..2,4-表油菜素内酯对干旱胁迫下夏枯草种子萌发与幼苗生理特性的影响[J].中国草地学报,2025,47(7):48-60,13.基金项目
河南省科技攻关项目(242102110158) （242102110158）
河南省科技研发计划联合基金项目(222103810057) （222103810057）
大学生创新创业训练计划项目(2024471,2025463) （2024471,2025463）
