中国草地学报2025，Vol.47Issue(7)：70-82,13.DOI:10.16742/j.zgcdxb.20240315
30份苜蓿种质苗期耐盐性综合评价
Comprehensive Evaluation of Salt Tolerance of 30 Alfalfa Germplasms at Seedling Stage
摘要
Abstract
To investigate salt tolerance in alfalfa(Medicago sativa)germplasm,30 alfalfa accessions of diverse origins were subjected to salt stress(200 mmol/L NaCl)for 15 days.Responses were evaluated through changes in seedling morphology,photosynthetic parameters,and physiological indices compared to control plants(CK,irrigated with Hoagland nutrient solution).The results demonstrated that NaCl treat-ment decreased plant height,leaf area,leaf relative water content,root activity,shoot dry weight,chloro-phyll a,chlorophyll b,transpiration rate,stomatal conductance,net photosynthetic rate,intercellular CO2 concentration,water use efficiency,catalase(CAT)activity,and potassium(K+)content relative to CK.Conversely,root dry weight,root-to-shoot ratio,stomatal limitation value,proline content,malondialde-hyde(MDA)content,peroxidase(POD)activity,superoxide dismutase(SOD),sodium(Na+)content,and the Na+/K+ratio exhibited increases.Regression analysis identified stomatal conductance,root activ-ity,root-to-shoot ratio,and Na+content as critical indicators for salt tolerance evaluation.The clustering analysis based on the comprehensive evaluation value of the membership function shows that the 30 alfalfa accessions were classified into four distinct tolerance categories:high salt-tolerant,moderately salt-tolerant,low salt-tolerant,and salt-sensitive.Accessions 7254 and 7657 exhibited high salt tolerance.关键词
苜蓿/耐盐性/聚类分析/回归分析Key words
Alfalfa/Salt tolerance/Cluster analysis/Regression analysis分类
农业科技引用本文复制引用
王利群,南丽丽,马香香,郭佳雨,田戈,何蓉..30份苜蓿种质苗期耐盐性综合评价[J].中国草地学报,2025,47(7):70-82,13.基金项目
甘肃省重大专项(25ZDNA005) （25ZDNA005）
财政部和农业农村部:国家现代农业产业技术体系(CARS-34) （CARS-34）
