中国草食动物科学2025，Vol.45Issue(4)：1-11,11.DOI:10.3969/j.issn.2095-3887.2025.00.010
不同海拔藏绵羊瘤胃微生物及宿主血清代谢组特征分析
Metabolomic Characterisation of Serum and Rumen Microbiota in Tibetan Sheep at Different Altitudes
摘要
Abstract
To explore the serum metabolic profiles and rumen microbiota metabolome characteristics of Tibetan sheep at different altitudes,18 healthy 3.5-year-old female Tibetan sheep were selected from three altitudes(2 500 m,3 500 m and 4 500 m),with 6 each group,and the rumen microbial and serum metabolites were determined by LC-MS/MS tech-nology.The results showed that 1 394 rumen microbial metabolites were identified in the three altitude groups of Ti-betan sheep.Among them,fructosyl-lysine content was significantly higher in the 3 500 m group than in the other two altitude groups(P<0.05),while L-kynurenine content significantly decreased with increasing altitude(P<0.05).KEGG functional annotation showed that the differential metabolites between the 3 500 m and 4 500 m groups were mainly en-riched in amino acid,lipid and carbohydrate metabolism pathways.The metabolites in the 3 500 m group were up-regulated,mainly concentrated in the vitamin B6 metabolism pathway,while the 4 500 m group was enriched in pyrimi-dine metabolism.A total of 3 582 metabolites were identified in the serum of Tibetan sheep at different altitudes.The differential metabolites between the 3 500 m and 4 500 m groups were mainly enriched in amino acid metabolism,lipid metabolism and cytochrome P450-mediated xenobiotic metabolism pathways,and were correlated with microbial me-tabolites.Interaction analysis showed that 274 common metabolites were identified in rumen fluid and serum,and these common metabolites were mainly enriched in metabolism-related pathways.In conclusion,the microbial metabolites and serum metabolites of Tibetan sheep were co-enriched in pathways related to lipid metabolism,energy metabolism and immune metabolism,which may be involved in the adaptive regulation of Tibetan sheep to high-altitude environ-mental changes.关键词
藏绵羊/瘤胃/微生物代谢物/血清代谢物/海拔Key words
Tibetan sheep/rumen/microbiota metabolites/serum metabolites/altitude分类
农业科技引用本文复制引用
马斌,王磊,陈小伟,沙玉柱,陈倩玲,杨文鑫,黄薇,刘秀..不同海拔藏绵羊瘤胃微生物及宿主血清代谢组特征分析[J].中国草食动物科学,2025,45(4):1-11,11.基金项目
甘肃省技术创新引导计划(24CXNG008) （24CXNG008）
