中国动脉硬化杂志2025，Vol.33Issue(6)：500-506,7.DOI:10.20039/j.cnki.1007-3949.2025.06.006
超声处理的聚苯乙烯纳米塑料对巨噬细胞脂质蓄积的影响
Effect of ultrasound treated polystyrene nanoplastics on lipid accumulation in macro-phages
摘要
Abstract
Aim To explore the effect of ultrasound-treated polystyrene nanoplastics(PS-NP)on lipid accumu-lation in macrophage-derived foam cells.Methods The CCK-8 method was used to detect the effects of PS-NP and high-frequency ultrasound-treated PS-NP(UPS-NP)on the activity of macrophages,oil red O staining and cholesterol de-tection kit were used to detect the intracellular lipid accumulation,RT-qPCR and Western blot were used to detect the ex-pression of mRNA and protein related to cholesterol uptake and efflux,as well as mRNA levels of cell burial related recep-tors.Results UPS-NP had no significant effect on the activity of macrophages,but UPS-NP could significantly in-crease the formation of macrophage-derived foam cells and increase the lipid accumulation in foam cells.UPS-NP could significantly upregulate the mRNA and protein expression of CD36 and scavenger receptor-A1(SR-A1),but did not affect the mRNA and protein expression of cholesterol efflux related receptors ATP-binding cassette transporter A1(ABCA1)and scavenger receptor-B1(SR-B1).UPS-NP did not affect the mRNA levels of receptors related to cell burial processes.Conclusion Ultrasound-treated PS-NP can significantly increase lipid accumulation in macrophage-derived foam cells,and its mechanism is related to the upregulation of CD36 and SR-A1 expression.关键词
聚苯乙烯纳米塑料/巨噬细胞/脂质蓄积/高频超声Key words
polystyrene nanoplastics/macrophage/lipid accumulation/high-frequency ultrasound分类
医药卫生引用本文复制引用
梁慧,王蕾,刘培庆,于杨..超声处理的聚苯乙烯纳米塑料对巨噬细胞脂质蓄积的影响[J].中国动脉硬化杂志,2025,33(6):500-506,7.基金项目
国家自然科学基金面上项目(81970385) （81970385）
山东省自然科学基金面上项目(ZR2019MH021) （ZR2019MH021）
中国博士后科学基金面上项目(2022M721336) （2022M721336）
泰安市科技发展计划引导项目(2020NS232) （2020NS232）
