中国动脉硬化杂志2025，Vol.33Issue(7)：587-594,8.DOI:10.20039/j.cnki.1007-3949.2025.07.005
PLIN2通过上调ACSL3表达促进THP-1源性巨噬细胞脂质蓄积
PLIN2 promotes lipid accumulation in THP-1 derived macrophages by upregulating ACSL3 expression
摘要
Abstract
Aim To investigate whether adipose differentiation-related proteins promote macrophage lipid accu-mulation by upregulating acyl-CoA synthetase long-chain family member 3(ACSL3)expression through PI3K/Akt.Methods The experiments were divided into 24 h group,different PLIN2 expression groups,HA-PLIN2+SC97 group and HA-PLIN2+LY294002 group.Western blot was used to detect the protein expression of PLIN2,Akt,p-Akt and ACSL3 in cells,RT-qPCR was used to detect the mRNA level of PLIN2 in cells,and oil red O was used to observe the degree of lipid accumulation in cells.Results The protein expression levels of Akt,p-Akt and ACSL3 in macrophages overex-pressing PLIN2 were significantly increased(P＜0.05),and p-Akt nuclear translocation was increased,with fluorescence labeling of PLIN2 and Akt overlapping.After adding the PI3K/Akt agonist SC97 to macrophages overexpressing PLIN2,the expression level of ACSL3 significantly increased(P＜0.05),and the degree of intracellular lipid accumulation in-creased;After adding the PI3K/Akt inhibitor LY294002 to macrophages overexpressing PLIN2,the expression level of AC-SL3 significantly decreased(P＜0.05),and the degree of intracellular lipid accumulation decreased.Conclusion PLIN2 upregulates ACSL3 expression through PI3K/Akt,thereby promoting macrophage lipid accumulation.关键词
脂肪分化相关蛋白/PI3K/Akt信号通路/巨噬细胞/脂质蓄积/酰基辅酶A合成酶长链家族成员3Key words
adipose differentiation-related protein/PI3K/Akt signaling pathway/macrophage/lipid accumu-lation/acyl-CoA synthetase long-chain family member 3分类
医药卫生引用本文复制引用
刘兰,杨丽,王语婷,刘新都,袁中华..PLIN2通过上调ACSL3表达促进THP-1源性巨噬细胞脂质蓄积[J].中国动脉硬化杂志,2025,33(7):587-594,8.基金项目
国家自然科学基金项目(81973326) （81973326）
