中国动脉硬化杂志2025，Vol.33Issue(7)：595-601,7.DOI:10.20039/j.cnki.1007-3949.2025.07.006
鸢尾素通过抑制内皮炎症减缓动脉粥样硬化的发生和发展
Irisin alleviates the occurrence and development of atherosclerosis by inhibiting endo-thelial inflammation
摘要
Abstract
Aim To investigate the effect of irisin on endothelial inflammation and atherosclerosis(As)in mice.Methods ApoE-/-mice were randomly divided into control group,As model group,and irisin group(treated with irisin based on the As model),with 10 mice in each group.The carotid tissues were stained using pathological techniques and immunofluorescence.Human aortic endothelial cells(HAEC)were cultured in vitro,treated with irisin,and stimulated with cholesterol crystal(CC).The protein levels of vascular cell adhesion molecule-1(VCAM-1)and intercellular cell adhesion molecule-1(ICAM-1)were then detected by Western blot.The expression of inflammatory cytokines interleukin-1β(IL-1β),interleukin-6(IL-6)and chemokine(C-C motif)ligand 2(CCL2)were detected by RT-qPCR.The ad-hesion of monocytes was assessed using cell adhesion assay.Results The carotid plaque area in the mice of As model group was significantly increased compared with that in control group(P＜0.05).In contrast,the plaque area was re-duced in the irisin group compared with the As model group(P＜0.05).Compared with the control group,the expression of VCAM-1,the number of CD68+macrophages,and the deposition of CC were increased in the carotid arteries of the As model group(P＜0.05),while irisin could reduce the expression of VCAM-1,the number of CD68+macrophages,and the deposition of CC(P＜0.05).At the in vitro level,the expression of VCAM-1 and ICAM-1,as well as the adhesion of monocytes in CC-stimulated HAEC,were increased(P＜0.05).However,irisin could inhibit the increased expression of VCAM-1 and ICAM-1(P＜0.05),as well as the adhesion of monocytes induced by CC(P＜0.05).The mRNA levels of IL-1β,IL-6 and CCL2 in HAEC of CC stimulated group were increased(P＜0.05),while irisin could inhibit the mRNA expressions of IL-1β,IL-6 and CCL2 induced by CC(P＜0.05).Conclusion Irisin can inhibit vascular inflamma-tion,thereby reducing the occurrence and progression of atherosclerosis.关键词
鸢尾素/动脉粥样硬化/内皮细胞/炎症Key words
irisin/atherosclerosis/endothelilal cell/inflammation分类
医药卫生引用本文复制引用
汪霞,周果,霍欢欢,何奔..鸢尾素通过抑制内皮炎症减缓动脉粥样硬化的发生和发展[J].中国动脉硬化杂志,2025,33(7):595-601,7.基金项目
国家自然科学基金项目(82130012和81830010) （82130012和81830010）
中德科学基金研究交流项目(M-0526) （M-0526）
上海市心律失常研究中心项目(2022ZZ01008) （2022ZZ01008）
SHDC重大疾病多中心临床研究项目(SHDC2020CR1039B) （SHDC2020CR1039B）
