局解手术学杂志2025，Vol.34Issue(7)：595-599,5.DOI:10.11659/jjssx.05E024103
雷公藤甲素对胃癌细胞增殖、迁移及侵袭的影响及机制研究
Effect of triptolide on proliferation,migration and invasion of gastric cancer cells and its mechanism
摘要
Abstract
Objective To explore the effects of triptolide(TPL)on the proliferation,migration and invasion of gastric cancer cells and its mechanism.Methods Human gastric cancer cell line MKN45 was cultured in vitro and treated with different concentrations of TPL for 48 hours.The cell proliferation inhibition rate was detected by CCK-8 method and the optimal concentration was selected for subsequent experiments.qRT-PCR was used to detect the expression of miR-29b and KDM2A mRNA in cells treated with different concentrations of TPL.MKN45 cells at logarithmic growth phase were randomly divided into the control group(without any treatment),the TPL group(treated with 200 µg/mL TPL),the inhibitor-NC+TPL group(transfected with inhibitor-NC and then treated with 200 µg/mL TPL),and the miR-29b inhibitor+TPL group(transfected with miR-29b inhibitor and then treated with 200 µg/mL TPL).qRT-PCR was used to detect the expression of miR-29b and KDM2A mRNA in each group of cells,and Western blot was used to detect the expression of KDM2A protein.The clone formation ability of each group of cells was detected by plate clone formation assay,and the migration and invasion abilities of each group of cells were detected by Transwell assay.Results TPL at concentrations of 25 µg/mL,50 µg/mL,100 µg/mL,and 200 µg/mL could significantly inhibit the proliferation of MKN45 cells(P<0.05),up-regulate the expression of miR-29b in cells(P<0.05),and down-regulate the expression of KDM2A mRNA(P<0.05).The effect was most obvious at the concentration of 200 µg/mL,so 200 µg/mL TPL was selected for the subsequent experiments.Compared with the control group,the expression of miR-29b in the TPL group increased(P<0.05),the expression of KDM2A mRNA and protein decreased(P<0.05),and the numbers of clone formation,migration and invasion cells reduced(P<0.05).Compared with the inhibitor-NC+TPL group,the expression of miR-29b in the miR-29b inhibitor+TPL group decreased(P<0.05),the expression of KDM2A mRNA and protein increased(P<0.05),and the numbers of clone formation,migration and invasion cells increased(P<0.05).Conclusion TPL can inhibit the proliferation,migration and invasion of gastric cancer cells,and its mechanism is related to the regulation of the miR-29b/KDM2A signaling pathway.关键词
胃癌/雷公藤甲素/miR-29b/KDM2A/细胞增殖/细胞迁移/细胞侵袭Key words
gastric cancer/triptolide/miR-29b/KDM2A/cell proliferation/cell migration/cell invasion分类
医药卫生引用本文复制引用
杜艳明,崔丽伟,檀靖宇,马召雨,马丽丽..雷公藤甲素对胃癌细胞增殖、迁移及侵袭的影响及机制研究[J].局解手术学杂志,2025,34(7):595-599,5.基金项目
河北省医学科学研究重点课题计划项目(20221814) （20221814）
