局解手术学杂志2025，Vol.34Issue(8)：663-668,6.DOI:10.11659/jjssx.10E024042
miR-205对结肠癌细胞增殖、迁移和上皮间质转化的调控作用研究
Regulatory effects of miR-205 on proliferation,migration,and epithelial-mesenchymal transition in colon cancer cells
摘要
Abstract
Objective To investigate the expression of microRNA-205(miR-205)in colon cancer and its regulatory effects on the proliferation,migration,and epithelial-mesenchymal transition(EMT)of colon cancer cells.Methods The expression levels of miR-205 in colon cancer tissues,adjacent normal tissues,and colon cancer cell HT-29 were detected by qRT-PCR.HT-29 cells were divided into three groups,cells transfected with miR-205 mimics were named the miR-205 mimic group,cells transfected with miR-205 inhibitors were named the miR-205 inhibitor group,and cells treated only with Lipo2000 transfection reagent were named the Buffer group.The proliferative activity of cells in each group was detected by CCK-8 assay and EDU assay;the migration invasion ability of cells was detected by Transwell assay;the expressions of EMT-related proteins N-cadherin,Vimentin,and E-cadherin in each group were detected by Western blot.Results Compared with adjacent normal tissues,the relative expression level of miR-205 in colon cancer tissues was significantly down-regulated(P<0.05).Compared with the Buffer group,the relative expression level of miR-205 in the cells of the miR-205 mimic group was up-regulated,while that in the miR-205 inhibitor group was down-regulated,the differences were statistically significant(P<0.05).Overexpression of miR-205 significantly inhibited the proliferation activity and migration ability of colon cancer cells(P<0.05),while inhibition of miR-205 promoted the proliferation activity and migration ability of colon cancer cells(P<0.05).Compared with the Buffer group,the levels of N-cadherin and Vimentin were down-regulated and the level of E-cadherin was up-regulated in the cells of the miR-205 mimic group,while the levels of N-cadherin and Vimentin were up-regulated and the level of E-cadherin was down-regulated in the cells of the miR-205 inhibitor group,the differences were all statistically significant(P<0.05).Conclusion The expression of miR-205 in colon cancer tissues is significantly decreased,and miR-205 exerts its tumor-suppressive effects by downregulating the proliferation,migration,and EMT of colon cancer cells.关键词
miR-205/结肠癌/细胞增殖/细胞迁移/上皮间质转化Key words
miR-205/colon cancer/cell proliferation/cell migration/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
买尔旦·赛力木,金钟,阿里旦·艾尔肯,柯于必..miR-205对结肠癌细胞增殖、迁移和上皮间质转化的调控作用研究[J].局解手术学杂志,2025,34(8):663-668,6.基金项目
新医大一附院"青年科研起航"专项基金(2022YFY-QKQN-15) （2022YFY-QKQN-15）
