中国临床药理学杂志2025，Vol.41Issue(11)：1532-1537,6.DOI:10.13699/j.cnki.1001-6821.2025.11.007
黄腐酚通过ROS和ERK信号通路对NG108-15细胞增殖的影响
Effects of Xanthohumol on proliferation of NG108-15 cells through ROS and ERK signaling pathways
摘要
Abstract
Objective To investigate the inhibition of NG108-15 cells proliferation by Xanthohumol(XN)and its effects on reactive oxygen species(ROS),extracellular signal-regulated kinase(ERK)pathway and cell cycle.Methods NG108-15 cells were divided into control group(conventional culture),experimental-L group,experimental-M group,experimental-H group(20,40,and 60 μmol·L-1 XN),NAC combined with XN experimental group[10 μmol·L-1 N-acetylcysteine(NAC)+40 μmol·L-1 XN]and ERK inhabitor combined with XN experimental group(20 μmol·L-1 ERK inhibitor PD98059+40 μmol·L-1 XN).Cell proliferation capacity was detected by cell counting kit 8(CCK-8)and trypan blue staining assay;ROS levels were detected by ROS kit;cell cycle and apoptosis levels were detected by flow cytometry;protein relative expression levels of ERK,p-ERK and Caspase 3 were detected by Western blot.Results The cell proliferation activities of the control group and the experimental-L group,experimental-M group and experimental-H group were(99.89±1.52)％,(87.57±6.23)％,(51.96±3.08)％and(37.11±1.57)％,respectively;the cell death rates were(4.65±1.57)％,(15.54±1.94)％,(41.06±2.77)％and(65.22±5.81)％,respectively;the average fluorescence intensities of ROS in the control group,the experimental-M group and the NAC combined with XN experimental group were 176.51±25.17,716.48±95.15 and 543.80±48.91.The relative expression levels of p-ERK protein in the control group,the experimental-M group,and the ERK inhibitor combined with XN experimental group were 0.54±0.04,1.13±0.18 and 0.31±0.33;the proportions of G2 phase cells were(15.56±1.43)％,(20.31±2.17)％ and(16.14±0.81)％,respectively.Compared with the control group,the above indicators in the experimental-L,-M,-H groups showed statistically significant differences(all P＜0.001).Compared with the experimental-M group,the control group and the NAC combined with XN experimental group showed statistically significant differences(P＜0.05,P＜0.01,P＜0.001).Compared with the experimental-M group,the ERK inhibitor combined with XN experimental group showed statistically significant differences(P＜0.05,P＜0.01,P＜0.001).Conclusion Xanthohumol demonstrates potent inhibition of NG108-15 cells proliferation,with its molecular mechanism likely involving G2/M phase cell cycle arrest through ROS upregulation and activation of the ERK signaling pathway.关键词
黄腐酚/脑胶质瘤/活性氧/细胞外调节激酶/细胞周期阻滞Key words
Xanthohumol/brain glioma/reactive oxygen specy/extracellular regulated protein kinase/cell cycle arrest分类
医药卫生引用本文复制引用
胡晨煜,田佳臻,闫涵宇,张婷,阿米尔·阿卜力孜,弥相权..黄腐酚通过ROS和ERK信号通路对NG108-15细胞增殖的影响[J].中国临床药理学杂志,2025,41(11):1532-1537,6.基金项目
石河子大学高层次人才科研启动基金资助项目(RCSX2018B01) （RCSX2018B01）
石河子大学校级基金资助项目(KX018904) （KX018904）
