中国临床药理学杂志2025，Vol.41Issue(11)：1538-1543,6.DOI:10.13699/j.cnki.1001-6821.2025.11.008
金福安汤调控PI3K/Akt/mTOR信号通路介导自噬对非小细胞肺癌细胞凋亡的影响
Effects of Jinfu'an decoction-mediates autophagy on apoptosis in non-small cell lung cancer cells by regulating PI3K/Akt/mTOR signaling pathway
摘要
Abstract
Objective To investigate the effect of Jinfu'an decoction on the apoptosis of non-small cell lung cancer cells(A549)by regulating phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)signaling pathway mediated autophagy.Methods A549 cells were randomly divided into blank group,control group,combined group and experimental-L,-M,-H groups.The blank group was treated with 10％ blank serum.The control group was treated with 20 μmol·L-1 PI3K specific activator(740Y-P).The experimental-L,-M,-H groups were treated with 5％,10％ and 20％ Jinfu'an decoction containing serum,respectively.The combined group was treated with 20％ Jinfu'an decoction serum+20 μmol·L-1740Y-P.Flow cytometry was used to detect the apoptosis rate.Western blot was used to detect the expression levels of microtubule-associated protein 1A/1B-light chain 3(LC3),sequestosome-1(p62),Akt and mTOR.Results The apoptosis rates of experimental-H group,combined group,control group and blank group were(31.48±1.35)％,(14.91±1.26)％,(3.30±0.45)％and(7.73±0.50)％,respectively;the relative expression levels of LC3 Ⅱ/Ⅰ protein were 1.13±0.10,0.77±0.06,0.31±0.04 and 0.50±0.04,respectively;the relative expression levels of p62 protein were 0.32±0.03,0.56±0.05,1.07±0.06 and 0.83±0.06,respectively;the relative expression levels of p-Akt/Akt protein were 0.33±0.04,0.80±0.07,0.98±0.09 and 0.52±0.03,respectively;the relative expression levels of p-mTOR/mTOR protein were 0.53±0.04,0.84±0.06,1.14±0.08 and 1.01±0.06,respectively.The above indexes of the experimental-H group were statistically different from those of the combination group and the blank group(all P＜0.05).Conclusion Jinfu'an decoction can promote the apoptosis of A549 cells,and its mechanism may be related to the inhibition of PI3K/Akt/mTOR signaling pathway and the activation of cell autophagy.关键词
金福安汤/非小细胞肺癌/凋亡/自噬/磷脂酰肌醇3-激酶/蛋白激酶B/西罗莫司靶蛋白信号通路Key words
Jinfu'an decoction/non-small cell lung cancer/apoptosis/autophagy/phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway分类
医药卫生引用本文复制引用
邢明远,陈学武,吴亭,林学英,符茗铨,王开裕..金福安汤调控PI3K/Akt/mTOR信号通路介导自噬对非小细胞肺癌细胞凋亡的影响[J].中国临床药理学杂志,2025,41(11):1538-1543,6.基金项目
海南省自然科学基金资助(822MS182) （822MS182）
海南省卫生健康科技创新联合基金资助项目(WSJK2025MS143) （WSJK2025MS143）
