摘要
Abstract
Objective To investigate the regulatory mechanism of plantamajoside(PMS)on the growth and metastasis of non-small cell lung cancer(NSCLC).Methods A549 cells were divided into control group(normal culture),model group(40 μg·mL-1 PMS),NC inhibitor group(40 μg·mL-1 PMS+NC inhibitor),miR-525-5p inhibitor group(40 μg·mL-1 PMS+miR-525-5p inhibitor),sh-NC group(40 μg·mL-1 PMS+miR-525-5p inhibitor+sh-NC)and sh-ubiquitin conjugating enzyme E2C(UBE2C)group(40 μg·mL-1 PMS+miR-525-5p inhibitor+sh-UBE2C).Transwell assay was used to evaluate the invasion ability of A549 cells after different treatments.Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay was used to evaluate the apoptosis of A549 cells after different treatments.At the animal level,mice were injected with A549 cell suspension(5 × 106/100 μL)to construct the NSCLC model.They were randomly divided into model-M group,PMS group(50 mg·kg-1 PMS),miR-525-5p inhibitor-M group(50 mg·kg-1 PMS+miR-525-5p inhibitor)and sh-UBE2C-M group(50 mg·kg-1 PMS+miR-525-5p inhibitor+sh-UBE2C).All groups were treated for 5 days with once a day.The size of the transplanted tumor was measured with a ruler every 7 days,and the transplanted tumor was removed and weighed on the 28th day.The number of Transwell invasions in control group,model group,NC inhibitor group,miR-525-5p inhibitor group,sh-NC group and sh-UBE2C group were 173.62±23.46,82.05±14.17,84.69±13.82,143.48±19.81,139.57±18.24 and 105.93±16.25,respectively;the apoptosis rates were(22.65±4.01)％,(53.34±8.93)％,(51.46±8.81)％,(37.52±5.86)％,(34.83±5.27)％ and(48.75±6.19)％,respectively.Compared in model group and control group,compared in miR-525-5p inhibitor group and NC inhibitor group,compared in sh-UBE2C group and sh-NC group,there were significant differences in the number of Transwell invasion and apoptosis rates(all P＜0.05).At the animal level,28 d tumor volumes of model-M group,PMS group,miR-525-5p inhibitor-M group and sh-UBE2C-M group were(966.30±176.53),(495.70±52.40),(841.10±121.25)and(623.80±105.85)mm3,respectively;the tumor weight on day 28 were(1.43±0.52),(0.75±0.21),(1.32±0.43)and(0.85±0.26)g,respectively.Differences of the tumor volume and weight between PMS group and model-M group,sh-UBE2C-M group and miR-525-5p inhibitor-M group were statistically significant at the 28 th day(P＜0.01,P＜0.001).Conclusion PMS can significantly inhibit the growth and metastasis of NSCLC by regulating the miR-525-5p/UBE2C axis.关键词
车前子苷/微小核糖核酸-525-5p/泛素结合酶E2C分子轴/非小细胞肺癌/生长/转移/机制Key words
plantamajoside/microRNA-525-5p/ubiquitin-conjugating enzyme 2C molecular axis/non-small cell lung cancer/proliferation/invasion/mechanism分类
医药卫生
