中国临床药理学杂志2025，Vol.41Issue(12)：1682-1687,6.DOI:10.13699/j.cnki.1001-6821.2025.12.006
LncRNA CBR3-AS1调节miR-874-3p/MAPK1轴对子宫内膜癌细胞增殖、凋亡和迁移的影响
Impacts of LncRNA CBR3-AS1 on proliferation,apoptosis and migration of endometrial carcinoma cells by regulating miR-874-3p/MAPK1 axis
摘要
Abstract
Objective To investigate the impacts of long non-coding RNA(lncRNA)carbonyl reductase 3 antisense RNA 1(CBR3-AS1)on proliferation,apoptosis and migration of endometrial carcinoma(EC)cells by regulating miR-874-3p/mitogen-activated protein kinase 1(MAPK1)axis.Methods The expression of CBR3-AS1,miR-874-3p in cancer tissues and adjacent tissues of EC patients were detected by quantitative real-time polymerase chain reaction(qRT-PCR).KLE cells were divided into normal group(normal culture),si-NC group(transfected with si-NC),si-CBR3-AS1 group(transfected with si-CBR3-AS1),si-CBR3-AS1+inhibitor-NC group(co-transfected with si-CBR3-AS1 and inhibitor-NC),and si-CBR3-AS1+miR-874-3p inhibitor group(co-transfected with si-CBR3-AS1 and miR-874-3p inhibitor).The expression of CBR3-AS1 and miR-874-3p in cells were detected by qRT-PCR.The cell proliferation was detected by methyl thiazolyl tetrazolium(MTT)assay.Apoptosis was detected by flow cytometry.Cell migration was detected by scratch test.The expression of MAPK1 protein was detected by Western blot.Results The relative expression levels of CBR3-AS1 in paracancerous tissues and EC cancer tissues were 1.00±0.01 and 1.62±0.14,respectively;the relative expression levels of miR-874-3p were 1.00±0.02,0.25±0.02,respectively.Compared with paracancerous tissues,the relative expression level of CBR3-AS1 in EC tissues was increased,and the expression level of miR-874-3p was decreased,the differences were statistically significant(all P＜0.05).The relative expression levels of CBR3-AS1 in normal group,si-NC group,si-CBR3-AS1 group,si-CBR3-AS1+inhibitor-NC group and si-CBR3-AS1+miR-874-3p inhibitor group were 1.00±0.01,1.01±0.10,0.32±0.03,0.34±0.02 and 0.35±0.03;the relative expression levels of miR-874-3p were 1.00±0.10,1.02±0.11,1.85±0.16,1.84±0.14 and 1.12±0.10;cell proliferation rates at 72 h were(100.00±11.02)％,(99.28±8.96)％,(62.59±4.85)％,(63.31±5.01)％and(85.93±7.36)％;the apoptosis rates were(9.97±1.15)％,(10.82±1.23)％,(37.57±3.91)％,(36.32±3.62)％and(22.24±2.73)％;the scratch healing rates were(57.42±3.56)％,(56.94±3.38)％,(17.42±1.15)％,(17.37±1.89)％and(36.92±2.58)％;the relative expression levels of MAPK1 protein were 1.58±0.13,1.56±0.11,0.53±0.05,0.51±0.04 and 0.97±0.09,respectively.The differences were statistically significant in the above indexes between si-NC group and si-CBR3-AS1 group,si-CBR3-AS1+miR-874-3p inhibitor group and si-CBR3-AS1+inhibitor-NC group(all P＜0.05).Conclusion Knocking down CBR3-AS1 may down-regulate the expression of MAPK1 by targeting miR-874-3p,there by inhibiting the proliferation and migration of EC cells and promoting their apoptosis.关键词
长链非编码RNA羰基还原酶3反义RNA1/微小RNA-874-3p/丝裂原活化蛋白激酶1轴/子宫内膜癌/增殖/凋亡/迁移Key words
LncRNA carbonyl reductase 3 antisense RNA 1/miRNA-874-3p/mitogen-activated protein kinase 1 axis/endometrial carcinoma/proliferation/apoptosis/migration分类
医药卫生引用本文复制引用
汤云,刘明明,孙雯,胡晓婧..LncRNA CBR3-AS1调节miR-874-3p/MAPK1轴对子宫内膜癌细胞增殖、凋亡和迁移的影响[J].中国临床药理学杂志,2025,41(12):1682-1687,6.基金项目
青岛市民生科技计划基金资助项目(19-6-49-nsh) （19-6-49-nsh）
