中国临床药理学杂志2025，Vol.41Issue(12)：1688-1692,5.DOI:10.13699/j.cnki.1001-6821.2025.12.007
蟛蜞菊内酯对ox-LDL刺激下人角质形成细胞衰老的影响
Effect of wedelolactone on senescence induced by ox-LDL in human keratinocytes
摘要
Abstract
Objective To explore the protective effects of wedelolactone(WEL)against oxidative damage in HaCaT cells.Methods HaCaT cells were randomly divided into control group(normal culture),model group[100 μg·mL-1 oxidized low-density lipoprotein(ox-LDL)],experimental-M group(100 μg·mL-1 ox-LDL+20 μmol·L-1 WEL)and experimental-H group(100 μg·mL-1 ox-LDL+40 μmol·L-1 WEL).Methyl thiazolyl tetrazolium(MTT)assay was used to detect cell survival rate;senescence β-galactosidase staining kit was used to detect cell senescence;Hoechst 33258 staining was used to detect the apoptosis of the cells;Western blot was used to detect the expression of senescence-related proteins in the cells.Results The cell survival rates of the control group,model group and experimental-M,-H groups were(100.00±4.49)％,(163.99±11.37)％,(96.43±6.32)％and(93.02±9.50)％,respectively;the proportions of cell senescence were(7.86±4.51)％,(25.42±5.13)％,(13.32±5.28)％and(9.71±2.37)％,respectively;the apoptosis rates were(5.36±1.22)％,(17.27±4.39)％,(12.89±2.49)％and(7.94±3.63)％,respectively;the relative expression levels of p21 were 0.79±0.33,1.68±0.65,1.09±0.45 and 0.75±0.31,respectively;the relative expression levels of p16 were 1.00±0.29,2.10±0.31,1.50±0.28 and 1.20±0.15,respectively.The above indexes of the model group were compared with the control group,and the above indexes of the experimental-M,-H groups were compared with the model group(P＜0.000 1,P＜0.001,P＜0.05).Conclusion WEL can inhibit oxidative damage in HaCaT cells,and its mechanism is related to the inhibition of senescence induced by the damage.关键词
蟛蜞菊内酯/衰老/氧化型低密度脂蛋白/氧化应激反应/人永生化角质形成细胞Key words
wedelolactone/senescence/oxidized low-density lipoprotein/oxidative stress/human immortal keratinocyte line分类
医药卫生引用本文复制引用
李汶静,刘素连,刘丹,陈杰,许静..蟛蜞菊内酯对ox-LDL刺激下人角质形成细胞衰老的影响[J].中国临床药理学杂志,2025,41(12):1688-1692,5.基金项目
广东省自然科学基金资助项目(2018A030313138) （2018A030313138）
广东省基础与应用基础研究基金企业联合基金资助项目(2021A1515220165) （2021A1515220165）
广东省临床用药研究基金资助项目(2022JZ33) （2022JZ33）
广东省药理学会触发器研究基金资助项目(GYLY2021008) （GYLY2021008）
