中国临床药学杂志2025，Vol.34Issue(6)：414-421,8.DOI:10.19577/j.1007-4406.2025.06.003
衢枳壳总黄酮对肥胖型哮喘模型小鼠肺损伤的改善作用及脂质代谢的影响
Effects of total flavonoids from Quzhou Aurantii Fructus on lung injury and lipid metabolism in obese asthma model mice
摘要
Abstract
AIM To explore the effect of total flavonoids from Quzhou Aurantii Fructus(TFQA)on lung injury and lipid metabolism in obese asthmatic mice and its pharmacological effects.METHODS Obese asthmatic mice sensitized with ovalbumin(OVA)were fed a high-fat diet(60％fat ratio).The experiment included a healthy control group,an obese control group,an obese asthma group,and a TFQA intervention group,with 8 male BALB/c mice in each group.The lung function indices of mice were detected.Leukocytes in broncho alveolar lavage fluid were determined by the cell classification counting method.Serum levels of interleukin-1β(IL-1β),interleukin-17A(IL-17A),interleukin-6(IL-6),interleukin-10(IL-10),leptin(LEP),adiponectin(ADPN),and free fatty acid(FFA)were determined by ELISA.Western blot assay was employed to test lipid-associated protein expression in liver tissues.Pathological changes in liver and lung tissues were observed using hematoxylin-eosin(HE)staining,while lipid accumulation in liver tissues was assessed via Oil Red O staining.RESULTS Compared with the healthy control group,the airway resistance(RI)and functional residual capacity(FRC)of the obese asthma mice increased,with statistically significant differences(P＜0.01).Pulmonary function had declined.Serum LEP and FFA levels increased,IL-10 and ADPN levels decreased,HE staining showed that the liver and lung tissue were seriously damaged,with many white vacuoles and hepatic cord disorders.Liver tissue fat accumulation increased,sterol regulatory element-binding protein-1c(SREBP-1c),CCAAT enhancer binding protein beta(C-EBP β)and fatty acid synthase(FAS)were up-regulated,with statistically significant differences(P＜0.01).The expression of peroxisome proliferator-activated receptor-α(PPAR-α)was downregulated,and the difference was statistically significant(P＜0.01).Compared with the obese asthma model group,the above effects of TFQA were improved after intervention in obese asthmatic mice.At the same time red fat droplets in mouse liver tissue were reduced,and liver tissue contained carnitine palmitoyltransferase-1(CPT-1),carnitine palmitoyltransferase-1(CPT-2),protein levels of triglyceride lipase(TGL),hormone sensitive lipase(HSL)/P-HSL and PPAR-α were increased,with statistically significant differences(P＜0.01).CONCLUSION TFQA improved lung function in obesity-asthma mice,with its mechanism possibly related to inflammatory response inhibition and regulation of lipid metabolism.关键词
衢枳壳总黄酮/肥胖型哮喘/炎症反应/脂质代谢/支气管肺泡灌洗液Key words
total flavonoids from Quzhou Aurantii Fructus/obese asthma/inflammatory response/lipid metabolism/broncho alveolar lavage fluid分类
医药卫生引用本文复制引用
刘小娟,方月娟,梅国花..衢枳壳总黄酮对肥胖型哮喘模型小鼠肺损伤的改善作用及脂质代谢的影响[J].中国临床药学杂志,2025,34(6):414-421,8.基金项目
衢州市重点科技攻关项目(编号2022K79) （编号2022K79）
