中国老年保健医学2025，Vol.23Issue(3)：45-50,6.DOI:10.3969/j.issn.1672-2671.2025.03.009
柴胡疏肝散通过肠肝轴调节肠道菌群改善NAFLD大鼠肠黏膜屏障的研究
Chaihu-Shugan-San regulates Gut-Liver axis to improve intestinal mucosal barrier in NAFLD rats
摘要
Abstract
Objective To investigate the mechanism of Chaihu-Shugan-San(CSS)in ameliorating non-alcoholic fatty liver dis-ease(NAFLD)with liver stagnation and Qi stagnation pattern through modulation of gut microbiota and enhancement of intestinal mucosal barrier function.Methods A rat model of NAFLD with liver stagnation and Qi stagnation pattern was established by high-fat diet feeding combined with limb binding.Ninety SPF-grade SD rats were randomly divided into a normal control group(15 rats)and a model preparation group(75 rats).After successful modeling,the model group rats were randomly divided into five subgroups:the NAFLD model group,the Western medicine group(fenofibrate 20mg/kg),the low-dose CSS group(2.5g/kg),the medium-dose CSS group(5g/kg),and the high-dose CSS group(10g/kg).The normal control group received saline administration by gavage daily for 8 weeks.Rats were sacrificed,and serum,liver,and colon tissues were collected for detection of serum biochemical parameters,liver histopathology,colonic immune markers,and gut microbiota composition(analyzed by 16S rRNA gene sequencing).Results CSS significantly reduced serum levels of TG,TC,LDL,ALT,and AST in NAFLD rats(P＜0.05).HE staining showed that CSS alleviated hepatic steatosis and reduced pathological injury,with decreased lipid droplets in hepato-cytes.Immunohistochemical analysis indicated that CSS increased the expression of occludin and ZO-1 proteins in the colon tis-sue.16S rRNA gene sequencing revealed that CSS modulated the diversity and composition of gut microbiota,showing similarity to the normal control group.Conclusion Chaihu-Shugan-San significantly ameliorates NAFLD with liver stagnation and Qi stagnation pat-tern by regulating gut microbiota and enhancing intestinal mucosal barrier function.关键词
柴胡疏肝散/非酒精性脂肪性肝病/肠道菌群/黏膜屏障/肠肝轴Key words
Chaihu-Shugan-San/non-alcoholic fatty liver disease/gut microbiota/intestinal mucosal barrier/Gut-Liver axis引用本文复制引用
何顺勇,郑琳,赖金枚,林燕玉,叶诚乾,乔建峰..柴胡疏肝散通过肠肝轴调节肠道菌群改善NAFLD大鼠肠黏膜屏障的研究[J].中国老年保健医学,2025,23(3):45-50,6.基金项目
福建省自然科学基金项目(编号:2021J01901),2023年福建省"创双高"临床重点专科(脾胃)项目(编号:闽卫医政函[2023]1163号) （编号:2021J01901）
