中国美容医学2025，Vol.34Issue(8)：1-6,6.
蔓柏生发方对雄激素性脱发小鼠Th1/Th2/Th17/Treg平衡的影响
Effect of Manbaishengfa Formula on Th1/Th2/Th17/Treg Balance in Androgenic Alopecia Mice
摘要
Abstract
Objective To investigate the regulatory effect of Manbaishengfa formula on the balance of T cell subsets Th1/Th2/Th17/Treg in peripheral blood of androgenic alopecia mice.Methods Testosterone propionate was injected subcutaneously into the shaving area on the back of mice to establish the model of androgenic alopecia.AGA mice were divided into blank group,model group,minoxidil tincture group and high/medium/low dose groups of Manbaishengfa formula.After 30 days,the growth of mouse back hair was observed and recorded.The peripheral blood of mice was taken,and the proportion of Th1/Th2/Th17/Treg subsets was detected by flow cytometry.The expression of cytokine in mouse skin was detected by RT-PCR.Results Manbaishengfa formula could promote hair growth in depilation area of AGA mice compared with model group.The proportion of Th1 and Th17 subsets in Manbai shengfa formula group was up-regulated,and the proportion of Th2 and Treg subsets was down regulated compared with AGA mice.In addition,the content of IFN-γ,IL-1β,IL-17A and IL-17F in skin tissue decreased and the content of IL-4,IL-10 and TGF-β increased in Manbaishengfa formula group in a dose-dependent manner.Conclusion Manbaishengfa formula can promote the hair growth of AGA mice,regulate the balance of Th1/Th2/Th17/Treg cell subsets in the peripheral blood,and normalize the distribution of T cell subsets in peripheral blood of AGA mice.关键词
雄激素性脱发(AGA)/Th1/Th2/Th17/Treg平衡/蔓柏生发方/免疫调节Key words
androgenic alopecia(AGA)/Th1/Th2/Th17/Treg/Manbaishengfa formula/immune regulation分类
医药卫生引用本文复制引用
肖常青,李振洁,彭丽倩,王雨诗,李燕红,黄久遂,梁碧华,赵铁,朱慧兰,卢月..蔓柏生发方对雄激素性脱发小鼠Th1/Th2/Th17/Treg平衡的影响[J].中国美容医学,2025,34(8):1-6,6.基金项目
广州市科技计划-市校(院)联合资助基础与应用基础研究项目(编号:2023A03J0948,2024A03J0481,2024A03J0424) （院）
