中国免疫学杂志2025，Vol.41Issue(7)：1559-1565,7.DOI:10.3969/j.issn.1000-484X.2025.07.004
TACE联合恩度对肝癌患者CD4+/CD8+T细胞、ALAS1及HIPPO-YAP信号通路的作用
Effects of TACE combined with Endostar on CD4+/CD8+T cells,ALAS1 and HIPPO-YAP signaling pathway in patients with hepatocellular carcinoma
摘要
Abstract
Objective:To investigate the effects of transhepatic arterial chemoembolization(TACE)combined with Endostar on CD4+/CD8+T cells,5-aminoketovalonate synthase 1(ALAS1)and HIPPO-YAP signaling pathway in patients with hepatocellular carcinoma(HCC).Methods:A total of 60 HCC patients admitted to Chengdu Third People's Hospital from January 2018 to December 2021 were enrolled and randomly divided into TACE treatment group(A)and TACE combined with Endostar treatment group(B),with 30 patients in each group.T lymphocyte,ALAS1 and indicators of HIPPO-YAP signaling pathway were observed in the two groups.Results:In group A,1 subject had complete remission,2 subjects had partial remission,2 subjects had stable remission,and 4 subjects had progress;in group B,2 subjects had complete remission,4 subjects had partial remission,4 subjects had stable remis-sion,and 1 subject had progress.The total effective rate in group B(33.33%)was significantly higher than that in group A(10.00%),with significant difference among groups(P=0.028).There were no significant differences in CD4+T cells,CD8+T cells and CD4+/CD8+T cells between the two groups before treatment(P=0.972,0.995,0.917).After treatment,level of CD4+T cells in the two groups was significantly increased(P<0.001),while level of CD8+T cells was significantly decreased(P<0.001).CD4+/CD8+T cells was significantly increased(P<0.001),and the changes in group B were significant compared with group A(P<0.001).There was no significant difference in content of ALAS1 between the two groups before treatment(P=0.975);after treatment,content of ALAS1 in liver cancer tissues of the two groups was significantly increased(P<0.001),which in group B was significantly higher than that in group A(P<0.05).Before treatment,there were no significant differences in mammalian STE20-like protein kinase 2(MST2),large tumor suppressor 1(LATS1)and Yes-associated protein(YAP)between the two groups(P=0.134,0.134,0.134).After treatment,mRNA relative expressions of MST2 and LATS1 were significantly increased(all P<0.001),while mRNA relative expression of YAP were significantly decreased(P<0.001),and changes of group B were significant compared with group A(all P<0.001).After treat-ment,there were 5 more cases of no adverse reactions in group B than in group A,and the total incidence of adverse reactions(16.67%)was significantly lower than that in group A(43.33%),with a significant difference between groups(P=0.024).Conclu-sion:TACE combined with Endostar has significant therapeutic effect on HCC patients,which can effectively regulate CD4+/CD8+T cells,promote ALAS1 secretion,and activate HIPPO-YAP signaling pathway.关键词
TACE/恩度/肝癌/CD4+/CD8+T细胞/ALAS1/HIPPO-YAP信号通路Key words
TACE/Endostar/Liver cancer/CD4+/CD8+T cells/ALAS1/HIPPO-YAP signaling pathway分类
医药卫生引用本文复制引用
蒋莎莎,王蒙,张军,何春容,王轩可,杨玲蓉..TACE联合恩度对肝癌患者CD4+/CD8+T细胞、ALAS1及HIPPO-YAP信号通路的作用[J].中国免疫学杂志,2025,41(7):1559-1565,7.基金项目
四川省卫生健康委员会课题(21PJ147). （21PJ147）
