中国免疫学杂志2025，Vol.41Issue(7)：1581-1585,1592,6.DOI:10.3969/j.issn.1000-484X.2025.07.007
千金子二萜醇通过TGF-β/Smad信号通路影响肾透明细胞癌小鼠免疫因子IL-7、IL-10及VEGF表达
Euphorbia Lathyrol effects expressions of immune factors IL-7,IL-10 and VEGF in renal clear cell neoplasias mice through TGF-β/Smad signaling pathway
摘要
Abstract
Objective:To investigate the effect of Euphorbia Lathyrol on expressions of IL-7,IL-10 and VEGF in renal clear cell neoplasias(RCCN)mice by regulating TGF-β/Smad signaling pathway.Methods:RCCN xenograft mice were established and divided into model group,Euphorbia Lathyrol group and Cisplatin group.Euphorbia Lathyrol group were given 20 mg/kg Euphorbia Lathyrol solution intragastric administration,model group were given 0.9%NaCl gastric feeding,Cisplatin group were given intraperito-neal injection of 4 mg/kg cisplatin solution,then mice weight and tumor volume were recorded.Expressions of TGF-β1,Smad2 and Smad4 were tracked down through Western blot.Expressions of immune factors IL-7 and IL-10 in serum of xenograft mice were detected by ELISA.Expression of VEGF in tumor mass was detected by IHC.Results:After 14 days of administration,compared with model group,the proliferation of tumor grafts in Cisplatin group and Euphorbia Lathyrol group was inhibited(P<0.05),and the expressions of TGF-β1,Smad2,Smad4 protein and VEGF were observably decreased(P<0.05),expressions of IL-7 in serum was increased,while level of IL-10 in serum was decreased(P<0.05).Conclusion:Euphorbia Lathyrol is able to reduce expressions of IL-7,IL-10,as well as VEGF in RCCN xenograft mice by impeding TGF-β1/Smad signaling transduction system.关键词
千金子二萜醇/肾透明细胞癌/TGF-β/Smad信号转导通路/IL-7/IL-10/VEGFKey words
Euphorbia Lathyrols/Renal clear cell neoplasias/TGF-β/Smad signal transduction systems/IL-7/IL-10/VEGF分类
医药卫生引用本文复制引用
宋圣佑,周磊,邰伦伟,潘世杰,蒋俊玲,赵俊峰..千金子二萜醇通过TGF-β/Smad信号通路影响肾透明细胞癌小鼠免疫因子IL-7、IL-10及VEGF表达[J].中国免疫学杂志,2025,41(7):1581-1585,1592,6.基金项目
河南省中医药科学研究专项课题项目(20-21ZY2216). （20-21ZY2216）
