中国农业科学2025，Vol.58Issue(14)：2793-2804,12.DOI:10.3864/j.issn.0578-1752.2025.14.007
蛹期干旱胁迫对韭菜迟眼蕈蚊交配及虱螨脲敏感性的影响
Effects of Drought Stress During the Pupal Stage on Mating Behavior and Sensitivity to Lufenuron in Bradysia odoriphaga
摘要
Abstract
[Objective]Drought stress during the entire developmental period of Bradysia odoriphaga significantly suppresses its population occurrence.However,the specific impact of drought stress applied solely during the pupal stage on its population remains unreported.This study aims to clarify the effects of pupal-stage drought stress on the mating behavior of B.odoriphaga and the sensitivity of its F1 generation to lufenuron.It further explores the regulatory mechanism of soil drought stress on the B.odoriphaga population,providing a theoretical basis for reducing chemical pesticide application in its control.[Method]Soil drought treatment(10%relative water content)and soil wet treatment(40%relative water content)were established.Differences in water loss rate,developmental duration,and adult longevity were measured under these soil humidity conditions.Mating behavior of emerged adults was observed through paired trials.The sensitivity of F1 2nd-instar larvae to lufenuron was determined using the stem-dip method.Additionally,detoxifying enzyme activities and the expression levels of related coding genes were measured.[Result]Drought stress during the pupal stage significantly shortened adult longevity but did not affect pupal developmental duration.Mating trials revealed that drought-stressed males exhibited a 78.70%decrease in courtship attempts per minute,a 76.44%reduction in courtship duration per minute,and a 78.57%decline in mating success rate.Under drought treatment,the water loss rates at 72 h were 36.12%for female pupae and 41.12%for male pupae,both significantly higher than those in the wet treatment group.The LC50 value for F1 2nd-instar larvae from the drought group(14.343 mg·L-1)decreased by 37.37%compared to the wet group(22.902 mg·L-1).The mortality rate of 2nd-instar larvae treated with 14.00 mg·L-1 lufenuron was 53.33%in the drought treatment group and 26.67%in the wet treatment group.The difference was significant.The activities of P450 detoxifying enzymes,glutathione S-transferase and carboxylesterase in drought treatment group were significantly lower than those in wet treatment group.The results of real-time fluorescence quantitative PCR showed that the expression levels of CarE2,CarE3,GSTd1,CYP6QE1 and CYP3356A in the drought treatment group were significantly lower than those in the wet treatment group.The expression levels of CarE1 and CYP6FU12 were not significantly different,and the expression of GSTd2 was significantly up-regulated.[Conclusion]Drought stress leads to significant water loss in pupae,shortens adult longevity,and inhibits mating behavior.The use of soil drought stress during pupal stage can inhibit the occurrence of B.odoriphaga population.At the same time,drought stress during pupal stage leads to the increase of the lethal rate of lufenuron to F1 larvae and the change of detoxifying enzyme activity in larvae,indicating that it is feasible to control B.odoriphaga with drought stress and chemical control.关键词
韭菜迟眼蕈蚊/干旱胁迫/交配行为/虱螨脲/解毒酶Key words
Bradysia odoriphaga/drought stress/mating behavior/lufenuron/detoxifying enzyme引用本文复制引用
刘文龙,常译方,李文宇,孙丽娟,郑长英..蛹期干旱胁迫对韭菜迟眼蕈蚊交配及虱螨脲敏感性的影响[J].中国农业科学,2025,58(14):2793-2804,12.基金项目
山东省蔬菜产业技术体系(SDAIT-05) （SDAIT-05）
