中国全科医学2025，Vol.28Issue(27)：3399-3409,11.DOI:10.12114/j.issn.1007-9572.2024.0424
基于蛋白质组学技术分析腹部推拿对失眠大鼠下丘脑的影响研究
Effect of Abdominal Massage on Hypothalamus of Insomnia Rats Based on Proteomics
摘要
Abstract
Background Insomnia is a common sleep disorder that severely affects patients'quality of life and physical and mental health.Abdominal massage,as a non-pharmacological therapy,has gradually attracted attention due to its definite therapeutic effect on insomnia and its safety with no significant side effects.However,the specific mechanism of abdominal massage in treating insomnia is still not clear.Objectives We sought to scrutinise the distinctive hypothalamic protein expressions in p-chlorophenylalanine(PCPA)-provoked insomnia models in rats via proteomics,along with exploring the efficacy of abdominal massage in combating insomnia via behavioural analysis.Methods From March 2022 to May 2022,18 healthy male Sprague-Dawley(SD)rats with a body weight of(225±5)g were selected.The SD rats were randomly divided into three groups:the blank group,the model group,and the treatment group,with six rats in each group.Insomnia was created via intraperitoneal administration of PCPA.The blank group was untreated while the model group received no intervention.The treatment group underwent abdominal massage,once a day for 12 minutes,for seven straight days.Observations were made on animal behaviour in each group.Proteomics technology identified differential protein expressions in the hypothalamus,followed by biological information analysis for Gene Ontology(GO)functional annotation and KEGG enrichment.Lastly,related differential proteins were validated by Western blotting.Results The sodium pentobarbital righting reflex test was conducted 24 hours after modeling.The results showed that compared with the blank group,the model group and the treatment group had prolonged latency to sleep and shortened sleep duration(P<0.05),indicating that the modeling was successful in both groups.After 7 days of treatment,the behavioral results showed that compared with the blank group,the model group had decreased body weight,increased spontaneous paw-raising frequency,and prolonged time for rats to find the platform(P<0.05).Compared with the model group,the treatment group had increased body weight,reduced spontaneous paw-raising frequency,and shortened time for rats to find the platform(P<0.05).Proteomic analysis indicated that progerin-1(Psen1)and creatine kinase(Ckm),linked to insomnia,were elevated post-modeling(P<0.05)and reduced post-treatment(P<0.05).γ-aminobutyric acid receptor subunit α-5(Gabra5)and histone deacetylase 4(Hdac4)were decreased post-modeling(P<0.05)and elevated post-treatment(P<0.05).GO and KEGG pathway enrichment analysis were found that these differentially expressed proteins are mainly involved in biological processes such as protein activation cascade,energy metabolism,inflammatory response and striated muscle tissue development.Neuroactive ligand-receptor interaction and hematopoietic cell lineage are the main signaling pathways of abdominal massage therapy.Western blotting validation of these four proteins confirmed three substances(Psen1,Ckm and Gabra5)displayed trends consistent with mass spectrometry.Conclusion The analysis of behavioral results and proteomics results indicates that abdominal massage can effectively improve insomnia symptoms in SD rats.It exerts its effects through the neuroactive ligand-receptor interaction and the hematopoietic cell lineage pathway.Psen1,Ckm,and Gabra5 are identified as potential key proteins.These findings provide a fundamental basis for the use of abdominal massage in the treatment of insomnia.关键词
入睡和睡眠障碍/失眠症/下丘脑/腹部推拿/蛋白质组学Key words
Sleep initiation and maintenance disorders/Insomnia/Hypothalamus/Abdominal massage/Proteomics分类
医药卫生引用本文复制引用
张红石,曲子涵,孙雪峰,王宇峰,丛德毓,张野..基于蛋白质组学技术分析腹部推拿对失眠大鼠下丘脑的影响研究[J].中国全科医学,2025,28(27):3399-3409,11.基金项目
国家自然科学基金项目面上项目(82074569) （82074569）
吉林省自然科学基金(YDZJ202301ZYTS109) （YDZJ202301ZYTS109）
国家自然科学基金青年科学基金项目(82405605) （82405605）
吉林省教育厅科学研究项目(JJKH20250637KJ) （JJKH20250637KJ）
