中国人兽共患病学报2025，Vol.41Issue(6)：567-573,7.DOI:10.3969/j.issn.1002-2694.2025.00.096
华南虎源产肠毒素型大肠杆菌的分离鉴定及其生物学特性研究
Isolation,identification,and biological characterization of enterotoxigenic Escherichia coli from a South China tiger
摘要
Abstract
This study was aimed at identifying the pathogenic bacteria responsible for the death of a young tiger at the Fujian Meihua Mountain South China Tiger Breeding Research Institute.Tissue samples from the lungs,liver,and intestines of the deceased tiger were collected,and the bacteria were cultured inasterile environment.The bacterial strains were characterized according to their morphological and molecular biological properties,including assessment of virulence genes and antibiotic resistance genes,mouse lethality tests,and antibiotic susceptibility evaluations.A predominant bacterial strain isolated from the liver of the deceased tiger was identified as enterotoxigenic Escherichia coli(ETEC)strain Tiger22513F.Phylogenetic analysis of the 16S rRNA gene revealed that the Tiger22513F strain exhibited close genetic similarity to the reference strain ETEC(MF919609.1),with 99.9%nucleotide similarity,and resided on the same evolutionary branch.The Tiger22513F strain contained 11 antibiotic resistance genes(tetA,sul1,sul3,cmlA,floR,blaTEM,blaSHV,blaCMY-2,qnrA,qnrS,and qnrD)along with five virulence genes(VT1,fyuA,tsh,iucD,and ST).Mouse lethality tests indicated significant pathogenicity toward mice,affecting primarily the lungs,liver,and intestines.Antibiotic susceptibility testing demonstrated that this strain exhibited resistance to various classes of beta-lactam antibiotics,as well as quinolones and aminoglycosides.This investigation successfully isolated a multi-drug resistant enterotoxigenic Escherichia coli strain with pronounced pathogenicity from the liver of a deceased tiger;thus providing valuable scientific insights for clinical diagnosis,as well as prevention and control measures,against ETEC infections in South China tigers.关键词
华南虎/产肠毒素型大肠杆菌/分离鉴定/致病性Key words
South China tiger/enterotoxigenic Escherichia coli/isolation and identification/pathogenicity分类
医药卫生引用本文复制引用
许静茹,吴晓萍,廖玉婷,林伟业,肖晓子亿,李雪瑾,陈腾腾,林锡潘,林开雄,范克伟,朱智豪,李雨琪,范思思,康娅莉,卓钰槟,黄玲珊,丘淑琪,薛羽希..华南虎源产肠毒素型大肠杆菌的分离鉴定及其生物学特性研究[J].中国人兽共患病学报,2025,41(6):567-573,7.基金项目
福建省第四批"创新之星"人才项目(No.闽科专函[2023]110号) （No.闽科专函[2023]110号）
福建省高校产学合作项目(No.2020N5012) （No.2020N5012）
福建省林业科技项目(No.2024FKJ06) （No.2024FKJ06）
龙岩市科技创新联合资金社会发展科技项目(No.2023LYF17119) （No.2023LYF17119）
新罗区产学研科技联合创新项目(No.2022XLXYZ009) Fourth Batch of The"Innovation Star"Talent Project in Fujian Province(Fujian Special Letter[2023]No.110) （No.2022XLXYZ009）
Industry-university Cooperation Project of Fujian Province(No.2020N5012) （No.2020N5012）
Fujian Forestry Science and Technology Project(No.2024FKJ06) （No.2024FKJ06）
Longyan Science and Technology Innovation Joint Fund for Social Development Science and Technology Project(No.2023LYF17119) （No.2023LYF17119）
Xinluo District Science and Technology Joint Innovation Project(No.2022XLXYZ009) （No.2022XLXYZ009）
