中国人兽共患病学报2025，Vol.41Issue(6)：574-582,9.DOI:10.3969/j.issn.1002-2694.2025.00.100
苏州市副溶血性弧菌耐药、血清型及分子特征分析
Drug resistance,serotypes,and molecular characteristics of Vibrio parahaemolyticus in Suzhou
摘要
Abstract
This study was aimed at studying the drug resistance,serotypes,and molecular characteristics of Vibrio parahaemo-lyticus(VP)in Suzhou,to provide basic data for the prevention and control of VP-related diseases.Drug susceptibility testing of 177 VP strains isolated from Suzhou City in 2023 was performed with the microbroth dilution method.Virulence genes,serotypes,and multi-locus sequence typing(MLST)were analyzed on the basis of whole genome sequencing results.The drug resistance rate of 177 VP strains was highest against cefazolin(100.00%),followed by ampicillin(77.97%),and polymyxine E(63.84%),and the multiple drug resistance rate was 53.67%.In clinical isolates,O10∶K4(37.41%)was the most abundant serotype,and was followed by O3∶K6(28.78%),and ST3 was the dominant ST type.The main virulence genes of clinical isolates were tlh+,tdh+,and trh-(79.86%),whereas the virulence genes in food isolates were all tlh+,tdh-,and trh-.Strains of the same serotype clustered together in the SNP phylogenetic tree.The environmental isolates showed no obvious dominant serotype or ST type.In Suzhou,VP has a high proportion of multi-drug resistance,the clinical isolates have prevalent serotypes and ST types,and most isolates carried virulence genes;there-fore,monitoring should be strengthened.关键词
副溶血性弧菌/全基因组测序/耐药/血清型/分子特征Key words
Vibrio parahaemolyticus/whole genome sequencing/drug resistance/serotypes/molecular characteristics分类
医药卫生引用本文复制引用
王小龙,邹文燕,朱莉勤,张梦寒..苏州市副溶血性弧菌耐药、血清型及分子特征分析[J].中国人兽共患病学报,2025,41(6):574-582,9.基金项目
National Natural Science Foundation of China(No.32370099) （No.32370099）
Suzhou Medical Key Discipline Project(No.SZXK202117) （No.SZXK202117）
Key Technologies(Research)for Prevention and Control of Major Diseases and Infectious Diseases in Suzhou(No.GWZX202202,No.GWZX202302) （Research）
Project of Science and Education for Promoting Health Development(No.QNXM2024058,No.QNXM2024061) 国家自然科学基金项目(No.32370099) （No.QNXM2024058,No.QNXM2024061）
苏州市医学重点学科项目(No.SZXK202117) （No.SZXK202117）
苏州市重大疾病、传染病预防和控制关键技术(研究)(No.GWZX202202,No.GWZX202302) （研究）
苏州市"科教强卫"青年项目(No.QNXM2024058,No.QNXM2024061) （No.QNXM2024058,No.QNXM2024061）
