中国人兽共患病学报2025，Vol.41Issue(6)：583-591,9.DOI:10.3969/j.issn.1002-2694.2025.00.102
山东某大型肉鸡屠宰场弯曲菌分布特征及传播规律研究
Epidemiological distribution characteristics and transmission patterns of Campylobacter in a Shandong broiler slaughterhouse
摘要
Abstract
This research investigated the contamination level,distribution of drug-resistant strains,and molecular epidemiologi-cal characteristics of Campylobacter,and further explored transmission pathways and prevention strategies.Cecum,chicken carcass,chicken product,and environmental samples,as well as swabs from workers'hands,were collected from a slaughterhouse in a large broiler group in the Jiaodong area between August 2023 and July 2024.Quantitative contamination assessment of Campylobacter in chicken carcasses and chicken products was performed.After microbial mass spectrometry identification,the representative strains of different links were selected for drug resistance testing and whole genome sequencing(WGS).On the basis of the sequencing results,the resistance genes,virulence genes,multilocus sequence typing(MLST),and phylogenetic characteristics of representative strains were analyzed.Homology comparisons were performed between isolates and strains from patients with diarrhea in the NCBI database.A total of 297 Campylobacter strains were isolated from 806 samples,and the overall detection rate was 36.85%.The detection rate of Campylobacter was highest in the evisceration process(47.33%),followed by the cutting process(35.64%).Overall,the Campylo-bacter detection rate first increased,then decreased,and subsequently increased.Drug sensitivity testing revealed that 90 isolates were resistant to nalidixic acid and ciprofloxacin,and 94.97%of isolates were resistant to tetracycline.WGS showed that both Campylo-bacter jejuni(C.jejuni)and Campylobacter coli(C.coli)carried many drug resistance and virulence genes.ST-14176 of C.jejuni was isolated for the first time herein.The predominant ST-8261 strain of C.jejuni and ST-860,ST-829,and ST-1586 strains of C.coli are known to cause human diarrhea.LOS expression genes associated with Guillain-Barré syndrome(GBS)were detected in both C.jejuni isolates from the slaughter chain and patients with GBS.Some strains exhibited close genetic relatedness to human-derived Campylo-bacter strains from the NCBI database.The detection rate of Campylobacter in the slaughterhouse first increased,then decreased,and subsequently increased,and the quantitative contamination level of each link was similar to the detection rate.Quantitative analysis of chicken carcasses/products revealed that the average bacterial load was highest in eviscerated carcasses(102.80 cfu/g),and the high-est amount of Campylobacter in chicken products reached 451.80 cfu/g.Abundant drug resistance genes and virulence genes were iden-tified,and the drug resistance genes were highly correlated with the drug resistance rate.Therefore,surveillance intensity and control measures for Campylobacter in slaughter processes should be strengthened.关键词
弯曲菌/耐药特征/全基因组测序/分子流行病学Key words
Campylobacter/drug resistance/whole genome sequencing(WGS)/molecular epidemiology分类
医药卫生引用本文复制引用
苗帅,赵格,张喜悦,王君玮,王娟,曲志娜,黄秀梅,王琳,刘俊辉,赵建梅,高玉斌,宋时萍,张思宇,刘娜..山东某大型肉鸡屠宰场弯曲菌分布特征及传播规律研究[J].中国人兽共患病学报,2025,41(6):583-591,9.基金项目
国家重点研发计划(No.2022YFC2303900) National Key Research and Development Program(No.2022YFC2303900) （No.2022YFC2303900）
