中国人兽共患病学报2025，Vol.41Issue(6)：592-596,5.DOI:10.3969/j.issn.1002-2694.2025.00.094
环青海湖地区鼠疫疫源地鼠疫菌单核苷酸多态性研究
Single nucleotide polymorphism typing of Yersinia pestis in natural plague foci around Qinghai Lake
摘要
Abstract
This study was aimed at determining the molecular characteristics of Yersinia pestis in the natural plague foci around Qinghai Lake through single nucleotide polymorphism technology,to lay a foundation for molecular epidemiological and source-tracing analysis of Y.pestis in this area.Using the whole genome sequencing technology,we obtained the whole genome sequences of 84 representative Y.pestis strains.Using the sequences of Y.pestis and Yersinia pseudotuberculosis IP32953 from the NCBI database as references,we compared and analyzed the 2 298 SNP loci of these strains.From 1957 to 2020,84 representative strains of Y.pestis from the natural plague foci around Qinghai Lake were divided into two clades:1.IN2 and 3.ANT1.The 1.IN2 clade was the characteristic population of Y.pestis throughout all epidemic years in this area.Additionally,analysis of the SNP distribution and hosts in the region indicated that the 1.IN2 clade was located in five counties except Wulan,whereas the 3.ANT1 clade was isolated from Himalayan marmot and dog in two counties.In conclusion,the population structure of SNP of Y.pestis in the natural plague foci around Qinghai Lake is relatively simple,and SNP analysis of Y.pestis provided a scientific basis for tracing plague epidemic sources and formulating plague prevention and control measures in this area.关键词
环青海湖地区/鼠疫疫源地/鼠疫菌/单核苷酸多态性Key words
Qinghai Lake region/natural plague foci/Yersinia pestis/single nucleotide polymorphism(SNP)分类
医药卫生引用本文复制引用
李胜,李伟,靳娟,何建,杨晓艳,柏吉祥,辛有全,张丽,张晓璐,杜文琪..环青海湖地区鼠疫疫源地鼠疫菌单核苷酸多态性研究[J].中国人兽共患病学报,2025,41(6):592-596,5.基金项目
国家自然科学基金(No.82260401) （No.82260401）
青海省科技厅基础研究计划(No.2024-ZJ-712) National Natural Science Foundation of China(No.82260401) （No.2024-ZJ-712）
Science and Technology Plan Projects in Qinghai Prov-ince(No.2024-ZJ-712) （No.2024-ZJ-712）
