中国人兽共患病学报2025，Vol.41Issue(6)：597-602,6.DOI:10.3969/j.issn.1002-2694.2025.00.104
基于全基因组测序技术分析扬州市黄金海岸沙门氏菌病原学特征
Etiological characteristics of Salmonella enterica serovar Goldcoast isolates from Yangzhou,according to whole-genome sequencing
摘要
Abstract
Salmonella enterica serovar Goldcoast isolates from Yangzhou were analyzed for assessment of their pathogenic and ge-nomic characteristics.From 2017 to 2023,eight strains were detected through foodborne disease surveillance and health check-ups.These strains exhibited resistance to two to seven antibiotic classes,and all strains bore a T57S mutation in the parC gene.Notably,50％(4/8)of the strains carried sulfonamide resistance genes(sul1 or sul3),and 37.5％(3/8)were positive for extended-spectrum beta-lactamase(ESBL)genes,including blaCTX-M-55 and blaTEM-1.Multilocus sequence typing(MLST)revealed that ST358(five strains)and ST2529(three strains)were the predominant sequence types,corresponding to CRISPR Ⅰ and Ⅱ groups,respectively.Single nucleotide polymorphism(SNP)phylogenetic analysis indicated a close genetic relationship between the case isolate Sal-2214 and strains from multiple regions,with a SNP difference≤20,thus suggesting potential inter-regional transmission.For example,one isolate from the stool of a 4-year-old child with diarrhea(Sal-2214)carried the beta-lactam resistance gene(blaCTX-M-55)and exhib-ited high resistance to cefotaxime(CTX)and ceftazidime(CAZ).These findings indicated that S.enterica Goldcoast emerged in Yang-zhou from 2022,and was characterized by multidrug resistance and a genetic relationship with strains from coastal regions.These find-ings highlight the need for enhanced resistance monitoring and trace-back studies to better understand the epidemiology and control the potential spread of this pathogen.关键词
黄金海岸沙门氏菌/多重耐药/全基因组测序/CRISPR分型/单核苷酸多态性Key words
Salmonella enterica serovar Goldcoast/multidrug resistance/Whole Genome Sequencing/CRISPR typing/single nucleotide polymorphism分类
医药卫生引用本文复制引用
徐娅雯,王艳,许蓉蓉,陆荣荣,英航宁,周乐..基于全基因组测序技术分析扬州市黄金海岸沙门氏菌病原学特征[J].中国人兽共患病学报,2025,41(6):597-602,6.基金项目
江苏省预防医学科研课题面上项目(No.Ym2023040) （No.Ym2023040）
扬州市卫生健康委科研项目(No.2023-2-33) （No.2023-2-33）
扬州市疾病预防控制中心本级课题(No.YZJK2023-3). Preventive Medicine Research Project of Jiangsu Province(No.Ym2023040) （No.YZJK2023-3）
Special Scientific Research Fund of Yangzhou Health Commission(No.2023-2-33) （No.2023-2-33）
Yangzhou Center for Disease Control and Prevention(YZJK2023-3). （YZJK2023-3）
