中国人兽共患病学报2025，Vol.41Issue(6)：603-608,6.DOI:10.3969/j.issn.1002-2694.2025.00.108
海分枝杆菌全菌体蛋白抗原的免疫性能研究
Evaluation of the immunogenic properties of whole-cell proteins isolated from Mycobacterium marinum
段弘扬 1王瑞欢 2李马超 2刘海灿 2万康林2
作者信息
- 1. 传染病溯源预警与智能决策全国重点实验室,中国疾病预防控制中心传染病预防控制所,北京 102206||中国疾病预防控制中心环境与健康相关产品安全所,北京 100021
- 2. 传染病溯源预警与智能决策全国重点实验室,中国疾病预防控制中心传染病预防控制所,北京 102206
- 折叠
摘要
Abstract
In this study,we aim to evaluate the immunogenicity of the whole-cell proteins isolated from Mycobacterium marinum(MM).The findings of this research may provide scientific basis for the development of new candidate tuberculosis vaccines.Both MM 95014 and Mycobacteriumtuberculosis(MTB)H37Rv cells were routinely cultured and collected.After inactivation,whole-cell proteins were extracted by low-temperature ultrasonic fragmentation and mixed with DDA/Poly:IC adjuvant.The mixture was administered to BALB/c mice subcutaneously with 50 mg/mouse twice with an interval of 10 days.Blood sample was collected before each immunization,as well as 10 days after the last immunization.Blood samples and spleen tissue extracts were measured for serum antibody potency and cytokine levels by an enzyme-linked immunosorbent assay.An in vitro growth inhibition assay(MGIA)was used to assess the ability of mouse bone marrow-derived macrophages,collected after immunization with MM whole-cell proteins,to inhibit MTB growth.A MTB whole mycobacterium proteome microarray was applied to analyze the interaction between the serum in MM immunization group and MTB proteins.Our results indicated that,compared with the adjuvant group,MM whole-cell proteins induced higher levels of Th1 cytokines(IL-2,IFN-γ,IL-12,TNF-α)in the mice.The IgG and IgM antibody titers induced by MM immunization group reached 1∶608 874 and 1∶304 437,respectively.The MGIA results showed that the MM immunization group was able to significantly inhibit MTB growth in vitro.The MTB proteome microarray results indicated that there were 226 and 324 cross-proteins with IgG and IgM antibodies,respectively.This study suggested that MM whole-cell proteins can induce high levels of cellular and humoral immune responses in mice and exhibit strong inhibition of MTB growth in vitro.The results may suggest potential application value of MM whole-cell proteins as a novel candidate vaccine for tuberculosis.关键词
非结核分枝杆菌/海分枝杆菌/结核分枝杆菌/交叉抗原/结核疫苗Key words
Nontuberculous mycobacteria/Mycobacterium marinum/Mycobacterium tuberculosis/cross-antigen/tuberculosis vaccine分类
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段弘扬,王瑞欢,李马超,刘海灿,万康林..海分枝杆菌全菌体蛋白抗原的免疫性能研究[J].中国人兽共患病学报,2025,41(6):603-608,6.
