中国人兽共患病学报2025，Vol.41Issue(6)：617-622,6.DOI:10.3969/j.issn.1002-2694.2025.00.097
鲍曼不动杆菌耐药基因与耐药表型的一致性分析
Analysis of consistency between resistance genes and resistance phenotypes in Acinetobacter baumannii
顾琳 1高艳 1赵剑虹 1张若曦1
作者信息
- 1. 北京市朝阳区疾病预防控制中心微生物检验科 100021
- 折叠
摘要
Abstract
his study was aimed at understanding the drug resistance of Acinetobacter baumannii,exploring the consistency between resistance genes and resistance phenotypes,and providing a basis for the rational use of antibiotics in clinical practice.A total of 88 strains of Acinetobacter baumannii were isolated from hospitals at tertiary level or above within the jurisdiction from 2021 to 2023,and subjected to drug resistance testing and whole genome sequencing.The original data were analyzed for the entire genome process with a Microobench pathogenic microorganism analysis workstation(without reference splicing),and resistance genes were predicted and annotated.The strains were subjected to multi-point sequence analysisand minimum spanning tree construction.Among 88 strains of Acinetobacter baumannii,carbapenem resistant strains(CRAB)accounted for 72.73%,and 98.44%of CRAB showed multidrug resistance.In the past three years,the resistance to multiple drugs has increased.The cgMLST analysis showed that the ST2 type accounted for 89.77%,and a new ST type was discovered.After cgMLST analysis,the minimum spanning tree was generated,and the genetic relationship of the same ST type was closer.The same ST2 type was further divided into four clusters.Binary logistic regression analysis conducted on resistance genes and resistance phenotypes revealed that resistance genes APH(3″)-Ib,armA,ADC-73,sul1,and sul2 positively correlated with resistance phenotypes.The ST2 type was the main Acinetobacter baumannii type,and showed high rates of multidrug resistance and carriage of multiple resistance genes.The drug resistance situation is severe.The consistency between common resistance genes and resistance phenotypes is good.Clinical management of Acinetobacter baumannii infection must be strengthened to prevent outbreaks and transmission.关键词
鲍曼不动杆菌/耐药性/耐药基因/一致性Key words
Acinetobacter baumannii/drug resistance/resistance genes/consistency分类
医药卫生引用本文复制引用
顾琳,高艳,赵剑虹,张若曦..鲍曼不动杆菌耐药基因与耐药表型的一致性分析[J].中国人兽共患病学报,2025,41(6):617-622,6.
