中国蔬菜Issue(7)：63-69,7.DOI:10.19928/j.cnki.1000-6346.2025.5017
西瓜ERECTA基因克隆、生物信息学与非生物胁迫下表达分析
Cloning,Bioinformatics and Expression Analysis of ERECTA Gene in Watermelon under Abiotic Stress
摘要
Abstract
In this study,employed bioinformatics methods to analyze the physicochemical properties,conserved domains,transmembrane domains,and secondary/tertiary protein structures of the watermelon ERECTA gene(gene ID:Cla97C05G096750).Additionally,phylogenetic tree clustering analysis was performed.The full-length CDS sequence of the watermelon ERECTA gene was cloned,and its tissue-specific expression patterns under abiotic stresses were investigated.The results revealed that the ERECTA gene spans 6 894 bp,containing 27 exons and 26 introns,and is located on chromosome 5(25 867 785-25 874 678 bp),encoding 991 amino acids.Phylogenetic analysis indicated that this amino acid sequence shares a close evolutionary relationship with melon and cucumber,while showing the most distant relationship with rice.qRT-PCR demonstrated that the ERECTA gene exhibited higher expression levels in female flowers and leaves,but the lowest in stems.Under salt stress,its expression was upregulated,whereas it showed an initial increase followed by a decrease under drought and cold stresses,suggesting its potential role in stress responses.This study provides a foundation for future functional investigations of this gene in watermelon and targeted improvement of key agronomic traits.关键词
西瓜/ERECTA基因/基因克隆/表达分析Key words
watermelon/ERECTA gene/gene cloning/expression analysis引用本文复制引用
孙翔宇,戴思慧,龚诗琦,罗玉松,张成桃,宋慧娟,孙龙俊,何长征,汤冰倩,孙小武..西瓜ERECTA基因克隆、生物信息学与非生物胁迫下表达分析[J].中国蔬菜,2025,(7):63-69,7.基金项目
国家西甜瓜产业技术体系项目(CARS-25),2023年湖南省智能农机装备创新研发项目(ZLSS2023) （CARS-25）
