中国蔬菜Issue(7)：92-100,9.DOI:10.19928/j.cnki.1000-6346.2025.5018
南瓜种质资源苗期根腐病抗性鉴定及抗性遗传分析
Inheritance,Germplasm Collection and Screening of Fusarium Root Rot Resistance during the Seedling Stage in Pumpkin
摘要
Abstract
Fusarium root rot,caused by F.solani in the soil,has been reported as a devastating disease in commercial pumpkin worldwide.An effective strategy for controlling this disease is the development of resistant varieties;however,very few commercial cultivars resistant to the pathogen are currently available.To find resistant genetic source of pumpkin against Fusarium root rot,the isolate of F.solani was identificated by PCR technique with specific primers,and 252 accessions were manually inoculated with F.solani in the seedling stage.Inheritance of resistance to Fusarium root rot in inbred line GFB130(Cucurbita moschata Duch.)was also studied with six generations(P1,P2,F1,F2,BC1P1 and BC1P2).The results show that the causal agent resulting in root rot in pumpkin was Fusarium solani f.sp.cucurbitae race 1 in Beijing.Out of the 252 accessions screened,4 showed high tolerant,7 exhibited moderately tolerant,59 were susceptible and 182 were very susceptible to Fusarium root rot.Resistant and susceptible accessions accounted for 4.4％and 95.6％,respectively.The genetic analysis results revealed that a single dominant gene conferred resistance to Fusarium root rot in GFB130(C.moschata)and its generation.These results will facilitate development of resistant varieties of pumpkin to control this disease.关键词
南瓜/根腐病/腐皮镰刀菌/种质鉴定/遗传分析Key words
pumpkin/Fusarium root rot/Fusarium solani/germplasm identification/inheritance analysis引用本文复制引用
张少环,张国裕,张帆,田佳星,李海真,吴瑞刚,张沙沙,耿丽华..南瓜种质资源苗期根腐病抗性鉴定及抗性遗传分析[J].中国蔬菜,2025,(7):92-100,9.基金项目
国家重点研发计划项目(2023YFF1000100),瓜类协同中心建设项目(XTCX202301),宁波市科技计划项目(2021Z006) （2023YFF1000100）
