中国水产科学2025，Vol.32Issue(5)：581-594,14.DOI:10.12264/JFSC2025-0027
草鱼cdk2的分子特征、表达调控及其蛋白与CDX2的相互作用
Molecular characteristics,expression regulation and its coding protein's interaction with CDX2 of cdk2 in Ctenopharyngodon idella
摘要
Abstract
To investigate the molecular characteristics,interaction,and nutritional regulation of CDK2 in grass carp(Ctenopharyngodon idella),we obtained the grass carp cdk2 sequence by cloning;its open reading frame is 897 bp,encoding 298 amino acids.It exhibited a high degree of sequence and structure conservation in the analyzed species.The results of codon bias and cluster analyses based on CDK2 suggested that the grass carp has the closest relationship with zebrafish.Compared with clustering based on cdk2 RSCU,a phylogenetic tree analysis based on CDK2 sequence was more consistent with the conventional species classification.Tissue expression analysis showed the highest cdk2 mRNA levels in blood(P＜0.05),liver,and spleen;molecular docking and yeast two-hybrid assays were employed to analyze the interaction between grass carp CDK2 and its downstream phosphorylated substrate protein CDX2.The results showed that grass carp CDK2 interacted with CDK2.Furthermore,the regulation of different inclusion levels of free amino acid CAA(free amino acids:lys and glu),dipeptide KE(lys-glu,KE),tripeptide GHK(gly-his-glu,GHK)on cdk2 expression were examined,and the results revealed that compared with the control and other treatment groups,cdk2 expression showed the highest expression in the 0.5％GHK group;cdk2 expression of the CAA groups was higher than those of the KE dipeptide group which showed similar amino acids as the CAA group.In summary,cdk2 expression in grass carp exhibits tissue specificity;CDK2 interacted with CDX2;and its expression was regulated by the inclusion level of amino acids and oligopeptides.This study provides new clues for studying the molecular characteristics of grass carp cdk2,exploring the regulatory mechanism of intestinal function in other teleost fish,and provides a theoretical basis for further study on the molecular regulating mechanism of cdk2 on transporting and absorption of amino acids and oligopeptides.关键词
草鱼/cdk2/CDX2/相互作用/营养调控Key words
grass carp(Ctenopharyngodon idella)/cdk2/CDX2/interaction/nutrient regulation分类
农业科技引用本文复制引用
何志敏,曾鹏,卿艳玲,罗雅楠,刘臻,罗君涵,王辰浩洋,唐翔,蒋然,王麒翔,刘晶,李艳芳,高星..草鱼cdk2的分子特征、表达调控及其蛋白与CDX2的相互作用[J].中国水产科学,2025,32(5):581-594,14.基金项目
国家自然科学基金项目(U21A2026731902345) （U21A2026731902345）
湖南省自然科学基金面上项目(2023JJ30075) （2023JJ30075）
湖南省教育厅重点项目(22A0597) （22A0597）
湖南省领军人才计划项目(2023RC1072) （2023RC1072）
湖南省现代农业产业技术体系项目(HARS-07). （HARS-07）
