中国水产科学2025，Vol.32Issue(5)：622-634,13.DOI:10.12264/JFSC2024-0383
三角帆蚌血淋巴细胞对插核刺激的响应及矿化功能
Responses of hemolymphocytes to nucleus-inserting stimulation and biomineralization function of Hyriopsis cumingii
摘要
Abstract
In order to elucidate the effect of nucleus insertion stimulation on hemolymphocytes and their function in pearl mineralization of the freshwater pearl mussel Hyriopsis cumingii,the present study explored the morphology and classification of hemolymphocytes,including four types by means of light and electron microscopy based on cellular complexity,cell size,and nucleo-cytoplasmic ratio.Giemsa staining and flow cytometry analysis showed that the granularity complexity of hemolymphocytes increased significantly,cell diameter became larger after nucleus insertion,the proportion of granulocytes increased significantly(P＜0.05),and the proportion of hyalinocyte decreased significantly(P＜0.05).The results of histological observation showed that a large number of hemolymphocytes gathered near the insertion site and granulocytes were recruited.Ca2+content in hemolymphocytes and serum continued to significantly increase 2,5,and 10 d after insertion(P＜0.05)and then significantly decreased 20 and 50 d after insertion(P＜0.05),which revealed that the number composition,distribution,and Ca2+level of hemolymphocytes all respond to nucleus insertion.In vitro calcium carbonate crystallization experiments showed that hemolymphocytes and serum were able to influence the morphology of calcite but did not change the crystal shape.In summary,hemolymphocytes respond rapidly to the stimulation of nucleus insertion and can regulate biomineralization.This study lays a theoretical foundation for further research on the regulatory role of hemo lymphocytes in shellfish biomineralization.关键词
三角帆蚌/血淋巴细胞/插核响应/生物矿化/Ca2+水平Key words
Hyriopsis cumingii/hemolymphocytes/nucleus-inserting response/biomineralization/Ca2+level分类
农业科技引用本文复制引用
孙钧林,陈逸文,陈晓峰,李家乐,白志毅,李文娟..三角帆蚌血淋巴细胞对插核刺激的响应及矿化功能[J].中国水产科学,2025,32(5):622-634,13.基金项目
国家重点研发计划项目(2022YFD2400105) （2022YFD2400105）
国家现代农业产业技术体系(CARS-49) （CARS-49）
国家自然科学基金项目(31872565). （31872565）
