中国水稻科学2025，Vol.39Issue(4)：543-551,9.DOI:10.16819/j.1001-7216.2025.240508
水稻粉质胚乳突变体we1的表型分析与基因定位
Phenotypic Analysis and Gene Mapping of a Floury Endosperm Mutant we1 in Rice
摘要
Abstract
[Objective]The study of floury endosperm mutants is helpful for elucidating the molecular mechanisms underlying rice grain quality formation.[Method]A stably inherited floury endosperm mutant we1(white endosperm 1)was isolated from a 60Co-irradiated mutant pool of japonica rice cultivar Nipponbare.Phenotypic characterization and physicochemical analyses of we1 were conducted.An F2 population derived from a cross between we1 and indica variety 9311 was used for fine mapping.[Result]Compared with the wild type,we1 exhibited white,shrunken endosperm with irregularly shaped and loosely packed compound starch granules.The mutant showed significant reductions in 1000-grain weight,plant height,total starch content,and amylose content,along with a marked increase in lipid content.Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene.For map-based cloning,we1 was crossed with 9311 and recessive F2 individuals were selected.The WE1 locus was initially mapped to chromosome 6 and fine-mapped to a 181-kb physical interval.Twenty-three open reading frames(ORFs)were predicted in this region.qRT-PCR analysis revealed altered expression levels of starch synthesis-related genes in the we1 mutant.[Conclusion]WE1 likely encodes a novel gene involved in starch synthesis,providing a foundation for further elucidating its molecular regulatory mechanisms in rice.关键词
水稻/粉质胚乳/淀粉/理化性质/精细定位Key words
rice/floury endosperm/starch/physicochemical property/fine mapping引用本文复制引用
朱建平,杨杰,李霞,李文奇,许扬,王芳权,陶亚军,蒋彦婕,陈智慧,范方军..水稻粉质胚乳突变体we1的表型分析与基因定位[J].中国水稻科学,2025,39(4):543-551,9.基金项目
国家自然科学基金资助项目(32201861) （32201861）
江苏省重点研发计划资助项目(BE2023362) （BE2023362）
江苏省种业振兴揭榜挂帅项目(JBGS(2021)008). （JBGS(2021）
