中国生物防治学报2025，Vol.41Issue(3)：520-529,10.DOI:10.16409/j.cnki.2095-039x.2025.02.025
防治中药材根腐病芽胞杆菌的筛选及效果评价
Screening and Control Effect of Bacillus on Root Rot of Chinese Herbal Medicines
摘要
Abstract
In order to provide biocontrol germplasm resources for the biological control of root rot disease of Chinese herbal medicines,three rhizosphere bacteria with strong antibacterial activity against Fusarium which were strains LHJS2-2、LHQS2-5 and YHQS2-7 were selected from more than 10 000 strains by plate antagonism method.The range of inhibition bands were 12-17 mm including Fusarium oxysporum 295,F.solani N18 and F.Verticillium 173.But the inhibition zone against F.moniliforme N19 was only 3～5 mm.16S rDNA sequence analysis combining with its the morphological characteristic and the physiological and biochemical test results indicated that strain LHJS2-2 was identified as B.pumilus,strain LHQS2-5 as B.velezensis and strain YHQS2-7 as B.atrophaeus.The results of greenhouse test showed that the preventive efficacy of strains LHJS2-2,LHQS2-5 and YHQS2-7 against root rot of Astragalus were 88.07%,85.66%and 76.73%,respectively.The seedling height,root length,aboveground biomass and underground biomass were significantly promoted including Astragalus,Scutellaria baicalensis and Silver Bupleurum.The field experimental results indicated that the control efficacy of Bacillus strains LHJS2-2,LHQS2-5 and YHQS2-7 against root rot of Astragalus were 41.37%,40.13%and 41.95%,respectively.The field preventive efficacy of Bacillus strains LHJS2-2,LHQS2-5 and YHQS2-7 against root rot of Codonopsis pilosula were 87.06%,95.03%and 87.06%,respectively.It has an important prospect for strains LHJS2-2,LHQS2-5 and YHQS2-7 to bio-control the root rot disease of Chinese herbal medicine.关键词
中药材/根腐病/芽胞杆菌/筛选/生物防治Key words
Chinese herbal medicines/root rot/Bacillus/screen/bio-control分类
农业科技引用本文复制引用
沙月霞,曾庆超,田兴芳,李云翔,徐瑾瑜..防治中药材根腐病芽胞杆菌的筛选及效果评价[J].中国生物防治学报,2025,41(3):520-529,10.基金项目
宁夏重点研发计划项目(2022BBF02031) （2022BBF02031）
宁夏自然科学基金项目(2024AAC02062) （2024AAC02062）
宁夏农林科学院科技攻关项目(NKYG-24-20) （NKYG-24-20）
宁夏青年拔尖人才培养工程 （）
